Abstract
An endo-beta-1,4-mannanase was isolated from digestive fluid of Pacific abalone, Haliotis discus hannai, by successive chromatographies on TOYPEARL CM-650M, hydroxyapatite, and TOYOPEARL HW50F. The abalone mannanase, named HdMan in the present paper, showed a molecular mass of approximately 39,000 Da on SDS-PAGE, and exhibited high hydrolyic activity on both galactomannan from locust bean gum and glucomannan from konjac at an optimal pH and temperature of 7.5 and 45 degrees C, respectively. HdMan could degrade either beta-1,4-mannan or beta-1,4-mannooligosaccharides to mannotriose and mannobiose similarly to beta-1,4-mannanases from Pomacea, Littorina, and Mytilus. In addition, HdMan could disperse the fronds of a red alga Porphyra yezoensis into cell masses consisting of 10-20 cells that are available for cell engineering of this alga. cDNAs encoding HdMan were amplified by polymerase chain reaction from an abalone-hepatopancreas cDNA library. From the nucleotide sequences of the cDNAs, the sequence of 1232 bp in total was determined and the amino-acid sequence of 377 residues was deduced from the translational region of 1134 bp locating at nucleotide positions 15-1148. The N-terminal region of 17 residues except for the initi...Continue Reading
References
Jan 25, 1977·Biochimica Et Biophysica Acta·M A Porzio, A M Pearson
Mar 1, 1990·The Journal of Applied Bacteriology·A Araujo, O P Ward
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Aug 1, 1967·Journal of Biochemistry·H Maeda, N Ishida
Aug 1, 1993·Bioscience, Biotechnology, and Biochemistry·I Yamaura, T Matsumoto
Mar 1, 1995·Applied and Environmental Microbiology·H StålbrandM Penttilä
Apr 1, 1996·Bioscience, Biotechnology, and Biochemistry·I YamauraT Kato
Feb 27, 1999·Journal of Bacteriology·I K CannR I Mackie
Dec 30, 1999·Journal of Bacteriology·Y Tamaru, R H Doi
Feb 2, 2000·Applied and Environmental Microbiology·A SunnaP L Bergquist
Apr 25, 2001·Applied and Environmental Microbiology·C M TangC F Thurston
Jul 24, 2001·Journal of Bacteriology·J NöllingD R Smith
Jul 27, 2001·The Journal of Experimental Zoology·M Charrier, C Rouland
Nov 2, 2001·Journal of Biotechnology·Bingze XuJan-Christer Janson
Feb 22, 2002·Acta Crystallographica. Section D, Biological Crystallography·Bingze XuJerry Ståhlberg
Mar 16, 2002·European Journal of Biochemistry·Bingze XuJan-Christer Janson
Feb 13, 2003·European Journal of Biochemistry·Ken-ichi SuzukiKiyoyoshi Nishita
Apr 1, 1965·Canadian Journal of Microbiology·E T REESE, Y SHIBATA
Dec 12, 2003·Carbohydrate Research·Eri ShimizuKiyoyoshi Nishita
Jun 1, 1990·World Journal of Microbiology & Biotechnology·K G Johnson
Citations
Apr 4, 2008·Applied Microbiology and Biotechnology·L R S Moreira, E X F Filho
Mar 6, 2009·Applied Microbiology and Biotechnology·Min ZhangYu-Zhong Zhang
Aug 6, 2010·Journal of Industrial Microbiology & Biotechnology·Pengjun ShiBin Yao
Dec 20, 2011·Journal of Industrial Microbiology & Biotechnology·Huiying LuoBin Yao
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew J KingSimon J McQueen-Mason
Aug 24, 2013·BMC Research Notes·Carolina Aguilera-GálvezRicardo Acuña-Zornosa
Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Makiko SakkaKazuo Sakka
Jun 19, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Takuya SatohTakao Ojima
Dec 20, 2012·Molekuliarnaia genetika, mikrobiologiia i virusologiia·Jun Li HuangGui Xue Wang
Dec 10, 2015·Bioscience, Biotechnology, and Biochemistry·Yasushi MasudaAtsuo Kimura
Nov 13, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuya KumagaiTakao Ojima
Mar 1, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Umme Afsari ZahuraTakao Ojima
May 24, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Umme Afsari ZahuraTakao Ojima
Nov 25, 2011·Enzyme and Microbial Technology·Do Young KimHo-Yong Park
May 19, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuya Kumagai, Takao Ojima
Jun 27, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jung Min SongYoun-Ho Lee
Jun 22, 2011·Biochimie·Mohammad Matiur RahmanTakao Ojima
Jul 20, 2011·Bioresource Technology·Do Young KimHo-Yong Park
Dec 17, 2016·Scientific Reports·Qianqian LyuWeizhi Liu
Jul 12, 2018·Marine Biotechnology·Han Ming GanStuart Linton
Aug 31, 2017·PloS One·André BuschYannick Pauchet
Nov 12, 2015·The Journal of Biological Chemistry·Shogo MochizukiTakao Ojima
Dec 25, 2016·The Journal of Biological Chemistry·Hui-Min QinMasaru Tanokura
Oct 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kimihiko MizutaniBunzo Mikami
Apr 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonio Trincone
Nov 5, 2020·International Journal of Biological Macromolecules·Xi ChenKui Wang
Oct 28, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Stuart M Linton
Feb 6, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Stuart M Linton
Aug 18, 2009·The Journal of Experimental Biology·Kentaro Sakamoto, Haruhiko Toyohara
Jul 20, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Umme Afsari ZahuraTakao Ojima
Aug 17, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mohammad Matiur RahmanTakao Ojima