Isolation and cultivation of candidate phyla radiation Saccharibacteria (TM7) bacteria in coculture with bacterial hosts.

Journal of Oral Microbiology
Pallavi P MurugkarF. Dewhirst

Abstract

The vast majority of bacteria on earth have not yet been cultivated. There are many bacterial phyla with no cultivated examples including most members of the Candidate Phylum Radiation with the exception of human oral isolates from the phylum Saccharibacteria. The aims of this research were to develop reproducible methods and validate approaches for the cultivation of human oral Saccharibacteria and to identify the conceptual pitfalls that delayed isolation of these bacteria for 20 years after their discovery. Oral samples were dispersed and passed through 0.2 µm membrane filters. The ultrasmall saccharibacterial cells in the filtrate were pelleted, inoculated into broth cultures of potential bacterial host cells and passaged into fresh medium every 2-3 days. Thirty-two isolates representing four species of Saccharibacteria were isolated in stable coculture with three species of host bacteria from the phylum Actinobacteria. Complete genome sequences were obtained for 16 isolates. Human oral Saccharibacteria are obligate bacterial parasites that can be stably passaged in coculture with specific species of host bacteria. Isolating these important members of the human oral microbiome, and many natural environments, requires abando...Continue Reading

References

Jul 1, 1991·Journal of Bacteriology·T M SchmidtN R Pace
May 3, 1990·Nature·S J GiovannoniK G Field
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Mar 1, 1973·Canadian Journal of Microbiology·J F HoenigerJ L Stokes
Mar 1, 1965·Journal of Theoretical Biology·E Zuckerkandl, L Pauling
Jul 1, 1981·Journal of Periodontology·P A MashimoR J Genco
Jan 24, 1998·Journal of Bacteriology·P HugenholtzN R Pace
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·E O Kajander, N Ciftçioglu
Apr 26, 2001·International Journal of Systematic and Evolutionary Microbiology·A WillemsM Gillis
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rosario GilAndrés Moya
Dec 27, 2002·The Journal of General and Applied Microbiology·Takashi IizukaAkira Hiraishi
Oct 9, 2003·Applied and Environmental Microbiology·Cleber C OuverneyDavid A Relman
Dec 6, 2003·International Journal of Systematic and Evolutionary Microbiology·J DownesW G Wade
Feb 10, 2004·Applied and Environmental Microbiology·J Kirk HarrisNorman R Pace
May 5, 2006·Applied and Environmental Microbiology·Ruth E LeyNorman R Pace
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yann MarcyStephen R Quake
Apr 17, 2008·Nature Reviews. Microbiology·Rudolf Amann, Bernhard M Fuchs
Jul 1, 2003·FEMS Microbiology Ecology·Fei-Xue Fu, P R F Bell
Jul 14, 2010·Database : the Journal of Biological Databases and Curation·Tsute ChenFloyd E Dewhirst
Jul 27, 2010·Journal of Bacteriology·Floyd E DewhirstWilliam G Wade
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·James H CampbellMircea Podar
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh
Dec 24, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xuesong HeWenyuan Shi
Feb 28, 2015·Nature Communications·Birgit LuefJillian F Banfield
May 11, 2015·Journal of Microbiological Methods·Maria V SizovaSlava S Epstein
Jun 18, 2015·Nature·Christopher T BrownJillian F Banfield

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA282954
PRJNA28295

Methods Mentioned

BETA
PCR
electron microscopy

Software Mentioned

anvi
compute similarity
HOMD
TREE
blastn
MEGA X
RNA
IQ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.