Abstract
Culture of human oligodendrocyte precursor cells (OPCs) can help understand the regulatory mechanism of differentiation and myelination of oligodendrocytes. However, existing culture methods have limitations, particularly the lack of a source of human donor tissue and high cost. We sorted cells with the A2B5(+)PSA-NCAM(-) phenotype from neurospheres instead of human donor tissues through immunomagnetic sorting and subsequently cultured the isolated cells in OPC medium. Of all the isolated cells, 15.69% were of the A2B5(+)PSA-NCAM(-) phenotype. More than 90% of the isolated OPCs expressed the OPC-specific markers O4, PDGFαR, and Sox10, and less than 5% of cells expressed GFAP and Tuj-1. After induction, the isolated cells had the capacity to differentiate into oligodendrocytes. Furthermore, the OPCs could be stably passaged in vitro for at least four generations and all the cells had high expression levels of O4 and Sox10 and very low expression levels of GFAP and Tuj-1; moreover, the cells had the capacity to differentiate into oligodendrocytes. After four passages, OPCs can proliferate at least 14 times above. In addition, in the presence of B27, only one cytokine, namely, bFGF, was sufficient to maintain proliferation, and th...Continue Reading
References
Jan 1, 1995·Experimental Brain Research·C N SvendsenS B Dunnett
Oct 1, 1993·Developmental Biology·A L Gard, S E Pfeiffer
Mar 1, 1996·Trends in Neurosciences·R H Miller
Apr 4, 1997·Science·R McKay
Jan 13, 1999·The Keio Journal of Medicine·A Nishiyama
Jan 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lars M BjorklundOle Isacson
Jan 18, 2003·Progress in Neurobiology·Martin Stangel, Hans-Peter Hartung
Jan 2, 2004·Nature Medicine·Martha S WindremSteven A Goldman
Jul 15, 2004·Glia·Shih-Chun Lin, Dwight E Bergles
Oct 19, 2004·Journal of Neuroscience Research·Yevgeniya I TarasenkoPing Wu
Nov 13, 2004·Glia·Gabriel I NistorHans S Keirstead
Dec 8, 2004·The American Journal of Pathology·Francesca RuffiniJack P Antel
Jun 25, 2005·Journal of Neuroscience Methods·Zhongshu YangAkiko Nishiyama
Oct 21, 2006·Stem Cells·Sang-Moon KangDong-Wook Kim
Apr 19, 2007·BMC Developmental Biology·Frederick G StrathmannMargot Mayer-Pröschel
Sep 30, 2009·Journal of Neurotrauma·Bo Wu, Xianjun Ren
Oct 17, 2009·Nature Protocols·Bao-Yang HuSu-Chun Zhang
Jan 14, 2010·European Neurology·Artemios K Artemiadis, Maria C Anagnostouli
Apr 21, 2010·PloS One·Margherita NeriAngela Gritti
Jun 12, 2010·Stem Cell Research·Maria SundbergSusanna Narkilahti
Aug 20, 2010·Journal of Neuropathology and Experimental Neurology·Qiao Ling CuiGuillermina Almazan
Aug 17, 2011·Neurorehabilitation and Neural Repair·Arshak R AlexanianDennis J Maiman
Nov 30, 2011·Stem Cells and Development·Qiao-Ling CuiGuillermina Almazan
Jun 30, 2012·Journal of Neuroscience Methods·Toros A DincmanScott R Whittemore
Aug 23, 2012·PloS One·Jenea M BinTimothy E Kennedy
Sep 19, 2012·Brain Research Bulletin·Anna M CampbellPatricia J McLaughlin
Jan 5, 2013·Journal of Visualized Experiments : JoVE·Maria Chiara G MonacoEugene O Major
Oct 8, 2013·Brain Research·Kenta KubotaYoshiyuki Konishi
Jan 23, 2014·Stem Cells and Development·Daniela Emi SuzukiOswaldo Keith Okamoto
May 3, 2014·New Biotechnology·Ken-Ichi IsobeSachiko Ito
May 9, 2014·Glia·Qiao Ling CuiJack P Antel
Citations
Oct 16, 2015·Cellular and Molecular Life Sciences : CMLS·Chi XuPing-Yee Law
Jun 7, 2016·Drug Discovery Today·Martin MadillUna FitzGerald
May 14, 2017·Journal of Cellular Physiology·Hany E MareiPatrizia Casalbore
Jul 10, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ellen FritscheJulia Tigges
Sep 30, 2017·Frontiers in Cellular Neuroscience·Volker KroehneMichell M Reimer
Jun 25, 2019·Frontiers in Cellular Neuroscience·Yuan ZhangXing Li
Oct 28, 2017·Frontiers in Pharmacology·Sissi DolciIlaria Decimo
May 3, 2021·Journal of Translational Medicine·Haipeng ZhouZuo Luan
Aug 27, 2021·Membranes·Priscila Elias Ferreira StrickerKatherine Athayde Teixeira de Carvalho