Isolation and culture of human oligodendrocyte precursor cells from neurospheres

Brain Research Bulletin
Yabin LuZuo Luan

Abstract

Culture of human oligodendrocyte precursor cells (OPCs) can help understand the regulatory mechanism of differentiation and myelination of oligodendrocytes. However, existing culture methods have limitations, particularly the lack of a source of human donor tissue and high cost. We sorted cells with the A2B5(+)PSA-NCAM(-) phenotype from neurospheres instead of human donor tissues through immunomagnetic sorting and subsequently cultured the isolated cells in OPC medium. Of all the isolated cells, 15.69% were of the A2B5(+)PSA-NCAM(-) phenotype. More than 90% of the isolated OPCs expressed the OPC-specific markers O4, PDGFαR, and Sox10, and less than 5% of cells expressed GFAP and Tuj-1. After induction, the isolated cells had the capacity to differentiate into oligodendrocytes. Furthermore, the OPCs could be stably passaged in vitro for at least four generations and all the cells had high expression levels of O4 and Sox10 and very low expression levels of GFAP and Tuj-1; moreover, the cells had the capacity to differentiate into oligodendrocytes. After four passages, OPCs can proliferate at least 14 times above. In addition, in the presence of B27, only one cytokine, namely, bFGF, was sufficient to maintain proliferation, and th...Continue Reading

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Citations

Oct 16, 2015·Cellular and Molecular Life Sciences : CMLS·Chi XuPing-Yee Law
Jun 7, 2016·Drug Discovery Today·Martin MadillUna FitzGerald
May 14, 2017·Journal of Cellular Physiology·Hany E MareiPatrizia Casalbore
Jul 10, 2018·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ellen FritscheJulia Tigges
Sep 30, 2017·Frontiers in Cellular Neuroscience·Volker KroehneMichell M Reimer
Jun 25, 2019·Frontiers in Cellular Neuroscience·Yuan ZhangXing Li
Oct 28, 2017·Frontiers in Pharmacology·Sissi DolciIlaria Decimo
May 3, 2021·Journal of Translational Medicine·Haipeng ZhouZuo Luan
Aug 27, 2021·Membranes·Priscila Elias Ferreira StrickerKatherine Athayde Teixeira de Carvalho

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