Isolation and diagnosis of Helicobacter pylori by a new method: microcapillary culture

World Journal of Gastroenterology : WJG
Adil M AllahverdiyevBekir S Kocazeybek

Abstract

To investigate the performance of the microcapillary culture method (MCM) in Helicobacter pylori (H. pylori) isolation and diagnosis. Microcapillary culture (MC), classical culture (CC), rapid urease (CLO) test, and histopathologic examination (HE) were performed with biopsy samples. Homogenized biopsy samples were loaded into capillary tubes and incubated for 48 h at 37 °C without providing a microaerophilic environment. Additionally, three or four loops of the homogenized sample were inoculated in a ready-to-use selective medium (Becton Dickinson, Helicobacter Agar, Modified) specific for the isolation of H. pylori and incubated at 37 °C in a microaerophilic atmosphere provided by CampyGen (Becton Dickinson, GasPack). Bacteria reproducing in microcapillary tubes were evaluated in an inverted microscope and also were evaluated after performing a CC with the content. Results obtained by CC, CLO test, and HE were compared with those of MC. The diagnostic performances of the methods used in this study were evaluated for specificity, sensitivity, positive predictive value (PPV), negative predictive value (NPV), and CI. H. pylori was found positive by CLO test + HE and/or CC culture in 26 patient antrum and corpus biopsy samples. I...Continue Reading

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Citations

Sep 24, 2015·Digestive Diseases and Sciences·Nathan S S Atkinson, Barbara Braden
Mar 8, 2016·Journal of Theoretical Biology·Andrew LeberJosep Bassaganya-Riera
Aug 5, 2015·Clinical Endoscopy·Heung Up Kim
Sep 16, 2015·Helicobacter·Andreas MentisFrancis Mégraud
Jun 29, 2018·Journal of Pathogens·Amin Talebi Bezmin Abadi

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