Isolation and expression analysis of tuberous root development related genes in Rehmannia glutinosa.

Molecular Biology Reports
Peng SunXianen Li

Abstract

As one kind of important modified storage organs, tuberous roots are attractive for their economic and biological values. Although considerable progresses have been made in the past, molecular information regarding the tuberous root development is still limited. In this study, we focused on the molecular profiling of the tuberous root development of Rehmannia glutinosa. Suppression subtractive hybridization technology was employed to compare gene expression between adventitious root and developing tuberous root. As a result, a tuberous root subtractive library was constructed and 199 development-related unique expressed sequence tags were identified, which represent different groups of genes involved in metabolism, protein synthesis, protein fate, cell fate, signaling, transcription and development, etc. In order to further validate the obtained result, 18 genes were selected for expression analysis and the genes most likely being involved in tuberous root development were discussed. Our present study provided the first molecular profiling of tuberous root development-related genes in Rehmannia glutinosa, which will establish the basis for future deciphering the tuberous root development mechanism.

References

Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Apr 12, 2000·The Plant Journal : for Cell and Molecular Biology·M C DornelasM Kreis
Sep 12, 2000·Current Opinion in Genetics & Development·L Kim, A R Kimmel
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·W LukowitzC R Somerville
Sep 13, 2001·Plant Physiology·J LiJ Chory
Feb 15, 2003·FEBS Letters·Min Kyoung YouJung Myung Bae
Nov 13, 2003·Current Opinion in Plant Biology·Yi LiSimon McQueen-Mason
Dec 8, 2004·Protein and Peptide Letters·Cláudia Regina Batista de SouzaJúlio Cézar de Mattos Cascardo
Jan 13, 2005·Plant Physiology·Sophia L StoneJudy Callis
Feb 11, 2005·Journal of Plant Physiology·Masaru TanakaMakoto Nakatani
Mar 17, 2005·Chromosoma·U Thomas Meier
Jun 29, 2006·Journal of Experimental Botany·José I de la FuenteVictoriano Valpuesta
Jan 16, 2007·Annals of Botany·Kate Dreher, Judy Callis
Apr 15, 2008·Journal of Ethnopharmacology·Ru-Xue ZhangZheng-Ping Jia

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Citations

Dec 4, 2012·International Journal of Molecular Sciences·Peng SunXianen Li

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