PMID: 8586618May 1, 1995Paper

Isolation and expression of a chicken DNA methyltransferase cDNA

Journal of Biochemistry
S TajimaK Nishimori

Abstract

A 0.5 kb fragment of chicken DNA methyltransferase cDNA was PCR-amplified using a set of degenerate primers. A clone harboring a 5 kb insert was isolated from a cDNA library by screening with the PCR-amplified cDNA fragment as a probe. The elucidated nucleotide sequence gave a 4,614 nucleotide open reading frame, and the predicted protein was highly homologous to the mouse and human DNA methyltransferases, especially in the amino acid sequence of the catalytic domain in the carboxyl-terminal region. The cysteine-rich region and Lys-Gly repeat first found in the mouse sequence were also conserved in chicken. However, about 250 amino acid residues in the amino-terminal portion of chicken DNA methyltransferase diverged from the amino-terminus of the mouse or human sequence. Northern blot analysis showed that the message of chicken DNA methyltransferase was expressed at high levels in the testis, in the lung and in Marek's virus-transformed chicken T-lymphoma cells. Expression of the chicken DNA methyltransferase in COS1 cells demonstrated that the enzyme is a so-called maintenance-type methylase. When poly(dG-dC)-poly(dG-dC) was used as the methyl acceptor, to provide a measure of de novo methylase activity, the Km value for S-ade...Continue Reading

Citations

Dec 6, 1996·The Journal of Biological Chemistry·J A YoderS B Baylin
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·M S HungC J Shen
Dec 13, 2002·The Journal of Biological Chemistry·James FlynnNorbert O Reich
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·D W HsuC K Shen
Oct 16, 1999·DNA Sequence : the Journal of DNA Sequencing and Mapping·L ChenM Kimura

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