Isolation and expression of a hypotensive and anti-platelet acidic phospholipase A2 from Bothrops moojeni snake venom

Journal of Pharmaceutical and Biomedical Analysis
Lucas B SilveiraAndreimar M Soares

Abstract

Phospholipases A(2) are important components of snake venoms, the basic isoforms have been more extensively studied than the acidic groups, maybe due to their higher toxicity. Trying to better understand the role of the acidic isoforms on the envenomation process, an acidic phospholipase A(2) was purified from Bothrops moojeni snake venom through two chromatographic steps (BmooPLA(2)). The enzyme showed a relative molecular mass of 13,601Da, pI 5.2, high phospholipase activity, bactericidal effect, moderate cytotoxic activity and was able to inhibit platelet aggregation. Moreover, BmooPLA(2) induced moderate in vivo edema and hypotensive effect. The 414bp cDNA encoding the BmooPLA(2) was cloned and expressed in Escherichia coli. The recombinant BmooPLA(2) showed phospholipase and inhibitory activities on platelet aggregation similar to those of the native protein. A comparative study between BmooPLA(2), the acidic (BthA-I) and basic (BthTX-II) PLA(2) from B. jararacussu venom showed that the effects of BmooPLA(2) and BthA-I-PLA(2) are similar. BmooPLA(2) is the first isolated and characterized non-myotoxic PLA(2) from B. moojeni snake venom. The recombinant PLA(2) can substitute the native toxin in studies aiming its biotechnol...Continue Reading

References

Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Dec 26, 2001·Acta Crystallographica. Section D, Biological Crystallography·Lichuan GuZhengjiong Lin
May 3, 2003·Toxicon : Official Journal of the International Society on Toxinology·Wen-Li YangAn-long Xu
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Andreimar M Soares, José R Giglio
Aug 6, 2004·Protein Expression and Purification·Patrícia G RobertoSuzelei C França
Jan 31, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rodrigo G StábeliAndreimar M Soares
Nov 1, 2006·Protein Science : a Publication of the Protein Society·Min-Yi Shen, Andrej Sali
Apr 26, 2007·Current Topics in Medicinal Chemistry·Silvana MarcussiAndreimar M Soares
Jan 8, 2008·Biochimica Et Biophysica Acta·Timo J NevalainenKieran F Scott
Oct 22, 2008·Toxicon : Official Journal of the International Society on Toxinology·Norival A Santos-FilhoAndreimar M Soares
Mar 31, 2009·Toxicon : Official Journal of the International Society on Toxinology·Catarina TeixeiraFernando Chaves
Aug 20, 2009·Protein and Peptide Letters·Juliana I dos SantosMarcos R M Fontes

❮ Previous
Next ❯

Citations

Mar 22, 2016·Toxicon : Official Journal of the International Society on Toxinology·J R AlmeidaS L Da Silva
Aug 15, 2015·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Danilo L MenaldoSuely V Sampaio
Nov 29, 2014·Pharmaceutical Biology·Nathalia Novak ZobioleFrancielle Tramontini Gomes de Sousa Cardozo
Jul 7, 2016·Toxicon : Official Journal of the International Society on Toxinology·Eliécer Jiménez-CharrisBruno Lomonte
Sep 27, 2018·Drug Development Research·José R AlmeidaSaulo L da Silva
Aug 3, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Orsolya PéterfiLászló Bába
Mar 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Chuchu ZhangDavid Julius
Aug 10, 2020·Toxicon : Official Journal of the International Society on Toxinology·Weslei da Silva AguiarAnita Mitico Tanaka-Azevedo
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jacinthe FrangiehCésar Mattei
Jul 25, 2021·Chemico-biological Interactions·Rodrigo Simões-SilvaAndreimar M Soares
Nov 6, 2018·International Journal of Biological Macromolecules·Edigar H V DiasFábio de Oliveira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.