PMID: 2671992Sep 1, 1989

Isolation and expression of the Pneumocystis carinii thymidylate synthase gene

Proceedings of the National Academy of Sciences of the United States of America
U EdmanD V Santi

Abstract

The thymidylate synthase (TS) gene from Pneumocystis carinii has been isolated from complementary and genomic DNA libraries and expressed in Escherichia coli. The coding sequence of TS is 891 nucleotides, encoding a 297-amino acid protein of Mr 34,269. The deduced amino acid sequence is similar to TS from other organisms and is most closely related to the enzyme from Saccharomyces cerevisiae with 65% identity. TS is found on a 330-kilobase-pair chromosome in P. carinii. While TS and dihydrofolate reductase reside on a single polypeptide chain in all protozoa studied to date, TS is not linked to dihydrofolate reductase in P. carinii. The TS gene shows the presence of four small intervening sequences, some of which interrupt the coding sequence in highly ordered structural regions of the protein. Heterologous expression of P. carinii TS in E. coli was accomplished by cloning the coding sequence into plasmid vectors under control of the lac and tac promoters. These constructs direct the synthesis of catalytically active enzyme to the extent of 2% of total soluble protein.

References

May 1, 1992·European Journal of Epidemiology·Eduardo Dei-CasD Camus
Jan 1, 1992·Parasitology Research·J WatanabeH Ikeda
Mar 21, 1998·International Journal for Parasitology·E S Kaneshiro
Jan 24, 1991·The New England Journal of Medicine·P D Walzer
Dec 24, 1992·The New England Journal of Medicine·H Masur
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J C EdmanD V Santi
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D M SchmatzM J Turner
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S NarasimhanE Spicer
Jan 1, 1992·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·James R StringerJ Watanabe
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·Melanie CushionM Yamada
Jul 1, 1996·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·J C CailliezEduardo Dei-Cas
Jul 25, 1992·Nucleic Acids Research·Y LiuM J Leibowitz
Nov 1, 1992·Molecular Microbiology·T D EdlindJ W Smith
Jul 1, 1991·The Journal of protozoology·D J WilliamsM Y Armstrong
Nov 1, 1993·The Journal of Eukaryotic Microbiology·James R StringerMelanie Cushion
May 1, 1994·The Journal of Eukaryotic Microbiology·G A Weinberg, P J Durant
May 1, 1994·The Journal of Eukaryotic Microbiology·S M SunkinJames R Stringer
Jan 1, 1995·The Journal of Eukaryotic Microbiology·S M Sunkin, James R Stringer
Jan 1, 1995·The Journal of Eukaryotic Microbiology·E MazarsD Camus
May 1, 1995·The Journal of Eukaryotic Microbiology·J C Meade, James R Stringer
Sep 1, 1996·The Journal of Eukaryotic Microbiology·E MazarsEduardo Dei-Cas
Sep 1, 1996·The Journal of Eukaryotic Microbiology·S LatoucheP Roux
Oct 23, 1998·The Journal of Eukaryotic Microbiology·C M DenisC Odberg-Ferragut
Jan 1, 1990·Annals of the New York Academy of Sciences·Melanie CushionJames R Stringer
Nov 1, 1994·Antimicrobial Agents and Chemotherapy·S T FurlongJ A Fishman
May 1, 1994·Antimicrobial Agents and Chemotherapy·G LudewigC Staben
Oct 31, 2008·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Jang-Jih Lu, Chao-Hung Lee
Jun 21, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Cécile-Marie Aliouat-DenisEduardo Dei-Cas
Oct 1, 1991·Protein Expression and Purification·D V SantiP J Greene
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·James R Stringer
Jan 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·A SukuraT Ilvesniemi
Mar 25, 2018·Contact Dermatitis·Tina LejdingMalin Engfeldt

Citations

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D J BzikJ Inselburg
Jan 1, 1986·Annual Review of Biochemistry·R A PadgettP A Sharp
Mar 25, 1985·Nucleic Acids Research·K TakeishiT Seno
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R W HonessR E Randall
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M BeverleyJ S Cordingley
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R GrumontD V Santi
Nov 1, 1986·Reviews of Infectious Diseases·J Mills
Apr 1, 1986·Journal of Medicinal Chemistry·A L PogolottiD V Santi
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F K ChuM Belfort
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H A de BoerM Vasser
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L W DeBoerE Braunwald

Related Concepts

Thymidylate Synthase
Alkalescens-Dispar Group
Gene Amplification
Genome
Saccharomyces cerevisiae allergenic extract
AMINO ACIDS/Proteins
Pneumocystis Infections
Citrullinemia
Promoter
DNA Library

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.