Isolation and gene expression analysis of single potential human spermatogonial stem cells

Molecular Human Reproduction
Kathrein von KopylowA-N Spiess

Abstract

It is possible to isolate pure populations of single potential human spermatogonial stem cells without somatic contamination for down-stream applications, for example cell culture and gene expression analysis. We isolated pure populations of single potential human spermatogonial stem cells (hSSC) without contaminating somatic cells and analyzed gene expression of these cells via single-cell real-time RT-PCR. The isolation of a pure hSSC fraction could enable clinical applications such as fertility preservation for prepubertal boys and in vitro-spermatogenesis. By utilizing largely nonspecific markers for the isolation of spermatogonia (SPG) and hSSC, previously published cell selection methods are not able to deliver pure target cell populations without contamination by testicular somatic cells. However, uniform cell populations free of somatic cells are necessary to guarantee defined growth conditions in cell culture experiments and to prevent unintended stem cell differentiation. Fibroblast growth factor receptor 3 (FGFR3) is a cell surface protein of human undifferentiated A-type SPG and a promising candidate marker for hSSC. It is exclusively expressed in small, non-proliferating subgroups of this spermatogonial cell type t...Continue Reading

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Citations

Sep 7, 2016·Stem Cells and Development·Makiko YamadaMarco Seandel
Jan 18, 2017·Molecular Human Reproduction·N NeuhausH R Schöler
Aug 26, 2020·Journal of Assisted Reproduction and Genetics·Nilgün YersalPetek Korkusuz
Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Pu-Yao ZhangYang Yu
Apr 5, 2018·Biology of Reproduction·Hiroshi Kubota, Ralph L Brinster

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