Isolation and gene expression analysis of Arabidopsis thaliana mutants with constitutive expression of ATL2, an early elicitor-response RING-H2 zinc-finger gene

Genetics
Mario Serrano, P Guzmán

Abstract

Genes with unstable transcripts often encode proteins that play important regulatory roles. ATL2 is a member of a multigene family coding highly related RING-H2 zinc-finger proteins that may function as E3 ubiquitin ligases. ATL2 mRNA accumulation occurs rapidly and transiently after incubation with elicitors of pathogen response. We screened 50,000 M(2) families from a line that carries a fusion of pATL2 to the GUS reporter gene and isolated five mutants, which we named eca (expresión constitutiva de ATL2), that showed constitutive expression of the reporter gene. One mutant exhibits a drastic stunted phenotype while the other four grow similarly to wild type. Two early chitin-induced genes and known pathogenesis-related genes such as NPR1, PAL, and CHS are activated in all the mutants whereas members of the ATL family and PR-1 and PDF2.1, which are markers of the salicylic acid (SA) jasmonate (JA) defense-response pathways, display differential expression between the mutants. These observations indicate that the ECA gene products may function in the early steps of an elicitor-response pathway, although some of them may function at other stages on the SA or JA defense-response pathways. Likewise, the fact that ATL2 and other m...Continue Reading

References

May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·K HahlbrockE Schmelzer
May 20, 1994·Cell·R A DietrichJ L Dangl
Jun 11, 1993·Annals of the New York Academy of Sciences·P S Freemont
Jun 1, 1996·Current Opinion in Structural Biology·K L Borden, P S Freemont
Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Ebel
Mar 6, 1999·Current Opinion in Plant Biology·P Reymond, E E Farmer
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F KlessigH Silva
Aug 17, 2000·Plant & Cell Physiology·G FellbrichT Nürnberger
Sep 22, 2000·Trends in Cell Biology·P K JacksonJ D Reimann
Sep 28, 2000·Cell·C A Joazeiro, A M Weissman
Jun 26, 2001·Science·B J StaskawiczJ E Galan
Jul 6, 2001·The Plant Journal : for Cell and Molecular Biology·J D ClarkeJ E Parker
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G FelixT Boller
Feb 16, 2002·Science·Cristina AzevedoPaul Schulze-Lefert
Feb 16, 2002·Science·Mark J AustinJane E Parker
Jun 5, 2002·The Plant Cell·John G TurnerAlessandra Devoto
Aug 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rodrigo A GutierrezPamela J Green
Aug 16, 2002·Current Opinion in Plant Biology·Katrina M Ramonell, Shauna Somerville
Aug 16, 2002·Current Opinion in Plant Biology·Barbara N Kunkel, David M Brooks
Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Bing ZhangGary Stacey
Apr 16, 2003·The Plant Journal : for Cell and Molecular Biology·Jane GlazebrookFumiaki Katagiri
Jul 23, 2003·Current Opinion in Plant Biology·Jyoti Shah
Aug 16, 2003·Science·Marc T NishimuraShauna C Somerville
Sep 16, 2003·Current Opinion in Plant Biology·Peter D HareNam-Hai Chua
Nov 18, 2003·Annual Review of Genetics·Zachary NimchukJeffery L Dangl
Sep 1, 2002·Molecular Plant Pathology·Katrina M RamonellShauna Somerville

❮ Previous
Next ❯

Citations

Jun 2, 2011·Molecular Genetics and Genomics : MGG·Yanze LiChengchao Zheng
Jan 13, 2005·Plant Physiology·Sophia L StoneJudy Callis
Feb 12, 2010·Plant Physiology·Yadong HuangSusan I Gibson
Jun 13, 2012·Plant Physiology·Daniel MarinoSusana Rivas
May 28, 2010·BMC Plant Biology·Chidananda Nagamangala KanchiswamyGen-ichiro Arimura
Nov 2, 2013·Planta·Waseem Shahri, Inayatullah Tahir
Apr 9, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H ZhouSylvester Ndeda
Sep 8, 2010·Biochemical and Biophysical Research Communications·Gen-Ichiro Arimura, Massimo E Maffei
Sep 14, 2007·Current Opinion in Plant Biology·Sophia L Stone, Judy Callis
Jun 24, 2006·The Plant Journal : for Cell and Molecular Biology·Jae-Heung KoKyung-Hwan Han
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Hanae KoiwaiYoko Nishizawa
Oct 17, 2014·The Arabidopsis Book·Judy Callis
Feb 24, 2015·The Plant Journal : for Cell and Molecular Biology·Miaomiao TianQi Xie
Jul 26, 2012·Plant Signaling & Behavior·Plinio Guzmán
Aug 28, 2010·Plant Signaling & Behavior·Gen-ichiro Arimura, Tatsuya Sawasaki
Sep 8, 2011·Plant Signaling & Behavior·Takeo SatoJunji Yamaguchi
Sep 22, 2010·Molecular Plant-microbe Interactions : MPMI·Ludivine LassoisHaissam Jijakli
Aug 1, 2019·Plant, Cell & Environment·Fa-Qing Xu, Hong-Wei Xue
Nov 14, 2017·Molecular Plant-microbe Interactions : MPMI·Catherine BlancMario Serrano

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.