Isolation and Genetic Analysis of Multidrug Resistant Bacteria from Diabetic Foot Ulcers

Frontiers in Microbiology
Shailesh K Shahi, Ashok Kumar

Abstract

Severe diabetic foot ulcers (DFUs) patients visiting Sir Sunderlal Hospital, Banaras Hindu University, Varanasi, were selected for this study. Bacteria were isolated from swab and deep tissue of 42 patients, for examining their prevalence and antibiotic sensitivity. DFUs of majority of the patients were found infected with Enterococcus spp. (47.61%), Escherichia coli (35.71%), Staphylococcus spp. (33.33%), Alcaligenes spp. (30.95%), Pseudomonas spp. (30.95%), and Stenotrophomonas spp. (30.95%). Antibiotic susceptibility assay of 142 bacteria with 16 antibiotics belonging to eight classes showed the presence of 38 (26.76%) isolates with multidrug resistance (MDR) phenotypes. MDR character appeared to be governed by integrons as class 1 integrons were detected in 26 (68.42%) isolates. Altogether six different arrays of genes (aadA1, aadB, aadAV, dhfrV, dhfrXII, and dhfrXVII) were found within class 1 integron. Gene cassette dhfrAXVII-aadAV (1.6 kb) was present in 12 (3 Gram positive and 9 Gram negative) isolates and was conserved across all the isolates as evident from RFLP analysis. In addition to the presence of class 1 integron, six β-lactamase resistance encoding genes namely bla TEM, bla SHV, bla OXA, bla CTX-M-gp1, bla CTX-...Continue Reading

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Citations

Sep 20, 2019·The International Journal of Lower Extremity Wounds·Prakhar Srivastava, Karthikeyan Sivashanmugam
Oct 13, 2017·Frontiers in Microbiology·Delphine ChainierMarie-Cécile Ploy
Mar 29, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Prakhar Srivastava, Karthikeyan Sivashanmugam

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Methods Mentioned

BETA
amputation
amputations
biopsy
PCR

Software Mentioned

ClustalW2
Quantity One
BLASTn
MEGA

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