Isolation and genetic analysis of pure cells from forensic biological mixtures: The precision of a digital approach

Forensic Science International. Genetics
Francesca FontanaA Berti

Abstract

Latest genotyping technologies allow to achieve a reliable genetic profile for the offender identification even from extremely minute biological evidence. The ultimate challenge occurs when genetic profiles need to be retrieved from a mixture, which is composed of biological material from two or more individuals. In this case, DNA profiling will often result in a complex genetic profile, which is then subject matter for statistical analysis. In principle, when more individuals contribute to a mixture with different biological fluids, their single genetic profiles can be obtained by separating the distinct cell types (e.g. epithelial cells, blood cells, sperm), prior to genotyping. Different approaches have been investigated for this purpose, such as fluorescent-activated cell sorting (FACS) or laser capture microdissection (LCM), but currently none of these methods can guarantee the complete separation of different type of cells present in a mixture. In other fields of application, such as oncology, DEPArray™ technology, an image-based, microfluidic digital sorter, has been widely proven to enable the separation of pure cells, with single-cell precision. This study investigates the applicability of DEPArray™ technology to foren...Continue Reading

Citations

Dec 5, 2017·Médecine sciences : M/S·François-Xavier LaurentLaurent Pène
Nov 7, 2019·The American Journal of Forensic Medicine and Pathology·Papanu Suttipasit
May 29, 2018·International Journal of Legal Medicine·Ruiyang TaoChengtao Li
Dec 15, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mariano Di TrapaniGianni Medoro
Sep 26, 2019·Frontiers in Immunology·Jian Hang LamTony Kiat Hon Lim
Dec 29, 2020·Science & Justice : Journal of the Forensic Science Society·Kaitlin HuffmanJack Ballantyne
Mar 11, 2020·Forensic Science International. Genetics·Julia BurrillNunzianda Frascione
Mar 4, 2021·Cancers·Amelia J RushtonR Charles Coombes
Mar 31, 2021·Scientific Reports·Davis R L WatkinsMichael A Marciano

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