Isolation and genomic characterization of the TUPLE1/HIRA gene of the pufferfish Fugu rubripes

Gene
R LlevadotM Pritchard

Abstract

In an effort to obtain a small genomic construct for the generation of a HIRA transgenic mouse, we have isolated and sequenced the Fugu TUPLE1/HIRA gene. We have compared the gene organization and the proteins encoded in pufferfish and human and also searched for conserved DNA sequences that might be important in gene regulation. The pufferfish gene spans approx. 9 kb, which is approx. 11 times smaller than the human gene, owing to the reduced size of the introns. Like its human counterpart, it is organized into 25 exons. The majority of the splice sites are in identical positions to those found in the human gene, however, for three internal exons the positions of the splice sites are not directly comparable. The coding regions are almost identical in size and show a high degree of similarity, especially at the amino and carboxy termini. Comparisons of 5' and 3' sequences failed to detect similarities or sequences involved in regulation.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·S AparicioS Brenner
Apr 1, 1996·Trends in Genetics : TIG·G ElgarS Brenner
Dec 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·R LlevadotM Pritchard

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Citations

Feb 22, 2011·Molecular Reproduction and Development·Zhan-Ke ZhaoYu-Feng Wang
Aug 26, 1998·Biochemical and Biophysical Research Communications·R LlevadotP Scambler
Jun 7, 2002·Molecular Cell·Dominique Ray-GalletGeneviève Almouzni
Jul 6, 2000·FEBS Letters·B VenkateshS Brenner
Aug 1, 1998·Genome Research·M Angrist

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