PMID: 6970592Jan 6, 1981Paper

Isolation and identification of 25-hydroxyvitamin D2 25-glucuronide: a biliary metabolite of vitamin D2 in the chick

Biochemistry
L W LeVanH F DeLuca

Abstract

The biliary metabolites of vitamin D2 obtained from chickens dosed with 3H-labeled vitamin D2 were investigated. Most of the biliary radioactivity migrated as charged compounds on diethylaminoethyl-Sephadex chromatography, and the charged fraction could be resolved into several components by reversed-phase high pressure liquid chromatography. A major charged metabolite was further purified by reversed-phase high-pressure liquid chromatography. This compound was found to be beta-glucuronidase sensitive and to yield 25-hydroxyvitamin D2 upon mild acid hydrolysis. The metabolite was converted first to the methyl ester and then to silylated and acetylated derivatives, which were subjected to mass spectrometry. The structure of the original metabolite was established as 25-hydroxyvitamin D2 25-beta-D-glucuronic acid. This 25-hydroxyvitamin D2 25-beta-glucuronide is a major biliary metabolite of vitamin D2 in the chick and may play a role in the chick's discrimination against vitamin D2.

Citations

Apr 1, 1986·Digestive Diseases and Sciences·J E LedgerJ E Compston
May 8, 2001·Journal of Gastroenterology and Hepatology·I G FinlayD L Morris
Jan 1, 1986·Journal of the American College of Nutrition·M Gascon-Barré
Feb 1, 1982·Journal of Pharmaceutical Sciences·K T Koshy
Oct 1, 1981·Clinical Biochemistry·H F Deluca
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laura Navarro SuarezSascha Rohn
Jul 23, 2019·Cell Biochemistry and Function·Carl Jenkinson
Feb 20, 2021·Poultry Science·Bahram ShojadoostShayan Sharif

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