Isolation and identification of a natural reassortant mammalian orthoreovirus from least horseshoe bat in China

PloS One
Lihua WangGuodong Liang

Abstract

Mammalian orthoreoviruses (MRVs) have a wide geographic distribution and can infect virtually all mammals. Infections in humans may be either symptomatic or asymptomatic. This study describes the isolation and identification of a natural reassortant MRV from least horseshoe bats (Rhinolophus pusillu) in China, referred to as RpMRV-YN2012. The RpMRV-YN2012 was obtained from urine samples of Rhinolophus pusillus by cell culture. Negative-staining electron microscopy revealed that RpMRV-YN2012 was a non-enveloped icosahedral virus with ∼75 nm in diameter. Polyacrylamide gel electrophoresis (PAGE) migration patterns of the genome segments showed that RpMRV-YN2012 contained 10 segments in a 3:3:4 arrangement. The whole genome sequence of RpMRV2012 was determined. The consensus terminal sequences of all segments of 5'-GCUAh…yUCAUC-3' (h = A, U or C; y = C or U) were similar to the MRV species within the genus Orthoreovirus. Its evolution and evidence of genetic reassortment were analyzed by sequence comparison and phylogenetic analysis. The results showed that RpMRV-YN2012 is a novel serotype 2 MRV that may have originated from reassortment among bat, human, and/or pig MRV strains which associated with diarrhea, acute gastroenteritis...Continue Reading

References

Jan 1, 1973·Archiv für die gesamte Virusforschung·G P Gard, I D Marshall
Apr 30, 2004·The Journal of Infectious Diseases·Kenneth L TylerTerence S Dermody
Nov 17, 2005·Journal of Neurovirology·John S Mackenzie
Jul 19, 2006·Clinical Microbiology Reviews·Charles H CalisherTony Schountz
Oct 17, 2006·Reviews in Medical Virology·Samson WongKwok-Yung Yuen
Dec 26, 2006·Vector Borne and Zoonotic Diseases·Wim H M van der PoelJohannes A Kramps
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kaw Bing ChuaLin-Fa Wang
Aug 21, 2007·Comparative Immunology, Microbiology and Infectious Diseases·Tsutomu OmatsuYasuhiro Yoshikawa
Sep 6, 2008·Microbiology and Molecular Biology Reviews : MMBR·Colin R ParrishPeter Daszak
Apr 10, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Peter ChengWilina Lim
May 23, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·J Michael Day
May 25, 2010·Archives of Virology·Linfeng DuChangchun Tu
Aug 2, 2011·Emerging Infectious Diseases·Louise A OuattaraFlorence Komurian-Pradel
Aug 31, 2012·Zoonoses and Public Health·D LelliP Cordioli
Oct 23, 2012·Journal of Virology·Yimin DaiYongchang Cao
Jan 1, 2011·Emerging Health Threats Journal·Ivan V KuzminCharles E Rupprecht

❮ Previous
Next ❯

Citations

Jun 10, 2015·Viruses·Robert HollingworthRoger J Grand
Oct 18, 2015·The Journal of General Virology·Xing-Lou YangZheng-Li Shi
Apr 28, 2016·Food and Environmental Virology·Emilio D'UgoLaura Mancini
Sep 1, 2016·Annual Review of Virology·David T S Hayman
May 28, 2020·Viruses·Lara CavicchioMaria Serena Beato
Sep 5, 2018·BMC Veterinary Research·Tina NagličAndrej Steyer
Jul 25, 2020·The Journal of General Virology·Hayato HarimaHirofumi Sawa
Feb 10, 2021·Transboundary and Emerging Diseases·Jingqiang RenShipeng Cheng

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
PCRs
electron microscopy
electrophoresis

Software Mentioned

MEGA
DNASTAR

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.