Isolation and identification of an actin gene from Babesia gibsoni

The Journal of Parasitology
Jinlin ZhouX Xuan

Abstract

Actin is a ubiquitous and highly conserved microfilament protein that is hypothesized to play a mechanical force-generating role in the unusual gliding motility of sporozoan zoites and their active penetration of host cells. We have identified and isolated an actin gene from a Babesia gibsoni cDNA library by random sequencing. The complete nucleotide sequence of the actin gene is 1,243 bp; a single open reading frame encodes a polypeptide of 377 amino acid residues. The deduced amino acid sequence showed a high homology with actins from other species, especially with reported apicomplexan protozoans. The antiserum against recombinant actin expressed in Escherichia coli recognizes a 42-kDa native protein, which is consistent with its expected size. Immunofluorescence and confocal microscopic observation revealed that the protein is diffusely distributed throughout the B. gibsoni parasites.

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Citations

Apr 1, 2008·The Journal of Parasitology·Masahiro YamasakiYoshimitsu Maede
Nov 28, 2006·Veterinary Parasitology·Jinlin ZhouXuenan Xuan
Jun 26, 2013·Experimental Parasitology·Youn-Kyoung GooXuenan Xuan
Dec 29, 2020·Veterinary Parasitology·Lavanya K VijaykumarManjunatha Reddy G Bayyappa

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Datasets Mentioned

BETA
AY898613

Methods Mentioned

BETA
electrophoresis

Software Mentioned

BLAST

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Babesiosis (ASM)

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.