PMID: 18720839Aug 30, 2008Paper

Isolation and identification of an alkaliphilic Bacillus flexus XJU-3 and analysis of its alkaline amylase

Wei sheng wu xue bao = Acta microbiologica Sinica
Jian ZhaoErkin Rahman

Abstract

To isolate and identify a new Bacillus strain capable of growing under highly alkaline conditions as alkaline amylase producers and to characterize its enzymatic properties. The isolates were sampled from alkaline sewage in Shihezi city, Xinjiang and screened by plating them on the amylase agar medium depending on the halo zone diameter. The alkaline amylase producer with best enzymatic activity was designated as XJU-3. XJU-3 was identified by its physiological and biochemical characteristics, 16S rDNA sequence homology, and the content of its major cellular fatty acids. XJU-3 was a Gram-positive, spore-forming, aerobic, motile rod alkaliphilic bacterium. It can grow at a broad range of pH (4.0-12.5) in Luria broth medium and its optimum growth was at pH 10 and 37 degrees C. Its NaCl tolerance was up to 15%. Its major cellular fatty acids were anteiso-C15:0 and iso-C15:0. Comparative 16S rRNA gene sequence analyses showed that XJU-3 was most closely related to Bacillus flexus, with 99% similarity. The genomic DNA (G+C) content of our isolate was 39.13 mol %. XJU-3 produced extracellular alkaline amylase, and its maximal enzyme activity was observed at 40 degrees C and pH 10.0. More than 70% of the enzymatic activity was remaine...Continue Reading

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