PMID: 9172400May 1, 1997Paper

Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction

Journal of Applied Microbiology
M D BrookP M Desmarchelier

Abstract

An environmental soil survey to detect Burkholderia pseudomallei was performed during the dry and wet seasons in Darwin, Northern Territory, Australia. Soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. Selective culture techniques using Ashdown's and Galimand and Dodin's methods and the polymerase chain reaction (PCR) using specific 16S rRNA primers were used to detect and identify the organism and determine its distribution within the soil stratum over the change in seasons. Results showed that Ashdown's method gave higher isolation rates in the dry season, and Galimand and Dodin's method gave higher isolation rates during the wet season. PCR of the soil enrichment proved to be a more sensitive method than culture and was also a useful confirmatory test in determining the identification of isolates where biochemical tests gave inconsistent results. The PCR primers were specific and able to detect 10(1) cfu g-1 soil and 10(4) cfu g-1 of soil using Ashdown's enrichment broth and Galimand and Dodin's broth, respectively. Overall the isolation of B. pseudomallei was greatest during the dry season and at the higher and lower soil d...Continue Reading

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Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·P TrivediH V Batra
May 6, 2011·Transactions of the Royal Society of Tropical Medicine and Hygiene·Trinh Thanh TrungIvo Steinmetz
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Apr 16, 2005·Clinical Microbiology Reviews·Allen C Cheng, Bart J Currie
Sep 19, 2006·Applied and Environmental Microbiology·Timothy J J Inglis, Jose-Luis Sagripanti
Feb 27, 2008·PLoS Neglected Tropical Diseases·Narisara ChantratitaSharon J Peacock
Nov 22, 2011·Applied and Environmental Microbiology·Direk LimmathurotsakulSharon J Peacock
Sep 26, 2013·The American Journal of Tropical Medicine and Hygiene·Andrew GoodyearSteven Dow

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