Isolation and identification of cholesterol esterase and pancreatic lipase inhibitory peptides from brewer's spent grain by consecutive chromatography and mass spectrometry.

Food & Function
Antonela G GarzónSilvina R Drago

Abstract

The isolation and identification of cholesterol esterase (CE) and pancreatic lipase (PL) inhibitory peptides obtained from the protein hydrolysate of brewer's spent grain (BSG) was performed. BSG peptides were fractionated and purified sequentially by anion exchange, gel filtration (FPLC), and reversed phase high-performance liquid chromatography (RP-HPLC). The fractions obtained from each chromatographic step were collected and the in vitro enzyme inhibitory activity was evaluated. The chromatographic purification process increased the in vitro activities. The most active fractions were evaluated using MALDI-TOF tandem mass spectrometry, which identified three peptides: a peptide with the highest CE inhibition capacity (WNIHMEHQDLTTME) and two peptides with PL inhibition capacity (DFGIASF and LAAVEALSTNG). These three peptides showed hydrophobic and acidic amino acid residues (Asp and Glu) and/or their amines (Asn and Gln), which could be a common feature among lipid-lowering peptides related to CE and PL enzyme inhibition. The in silico studies showed that the three peptides had high hydrophobicity and were susceptible to enzymatic hydrolysis performed by trypsin, pepsin, and pancreatin. The BSG byproduct was a good source of...Continue Reading

References

Mar 21, 2008·Biochemical and Biophysical Research Communications·Damien Yann ColinBrigitte Kerfelec
Aug 14, 2013·Journal of Agricultural and Food Chemistry·Huijuan ZhangWallace Yokoyama
Sep 29, 2015·Journal of Agricultural and Food Chemistry·Shanshan WangYuan Li
Jan 6, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qin ZhangBeiwei Zhu
Mar 21, 2018·Advances in Food and Nutrition Research·Shixiang YaoChibuike C Udenigwe
Jul 12, 2018·Plant Foods for Human Nutrition·Raúl E CianSilvina R Drago

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Methods Mentioned

BETA
gel filtration

Software Mentioned

UWM
BIOPEP
BIOPE
dock
FlexPepDock
PepDraw
- UVW
CABS
Shimadzu LC
STATGRAPHICS Centurion XV

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