Isolation and identification of cryptic bioactive regions in bovine achilles tendon collagen.

The Protein Journal
Pradipta BanerjeeC Shanthi

Abstract

Several proteins are known to host specific regions within their sequence, that when exposed or excised out proteolytically can display a range of physiological activities quite different from that of the parent protein. Collagen, a class of structural biopolymers and an important component of the extracellular matrix, is now known to harbor several such bioactive peptides which can act as physiological regulators. This study was undertaken to identify such cryptic sites from bovine Achilles tendon collagen and an antioxidative assay was used to screen for bioactivity. Bacterial crude protease was used to hydrolyze collagen and the hydrolysate was subjected to separation through ion-exchange column chromatography. Fractions were screened using conventional antioxidative assays and further purified by gel permeation chromatography. Two biologically active cryptic peptides were obtained displaying high antioxidative properties, E1 and F3. At low concentrations, both peptides displayed higher chelating ability than EDTA and were able to reduce the auto-oxidation of unsaturated fatty acid. The molecular weights of the peptides were found out through column chromatography and Tricine SDS PAGE; both displayed molecular mass below 4 k...Continue Reading

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Citations

Jan 30, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Pradipta BanerjeeC Shanthi
Feb 18, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Pradipta BanerjeeC Shanthi
Nov 12, 2014·Amino Acids·Pradipta BanerjeeC Shanthi
Dec 3, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Evaldo Cardozo de SouzaRenata Cristina Ferreira Bonomo
Mar 23, 2018·Critical Reviews in Biochemistry and Molecular Biology·Federica IavaroneTiziana Cabras
Aug 17, 2019·Frontiers in Cardiovascular Medicine·Sinead A O'RourkeMichael G Monaghan
Jan 9, 2020·BioMed Research International·Germán R Jiménez-GastélumErika L Silva-Benítez
Jul 27, 2021·Journal of Food Biochemistry·K Sivaraman, C Shanthi

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