Isolation and Identification of Dipeptidyl Peptidase IV-Inhibitory Peptides from Trypsin/Chymotrypsin-Treated Goat Milk Casein Hydrolysates by 2D-TLC and LC-MS/MS

Journal of Agricultural and Food Chemistry
Ying ZhangShangwu Chen

Abstract

New dipeptidyl peptidase IV (DPP-IV)-inhibitory peptides from trypsin/chymotrypsin-treated goat milk casein hydrolysates were isolated and identified by two-dimensional silica thin-layer chromatography (2D-TLC) combined to nano LC-MS/MS. 2D-TLC with chloroform/methanol/25% ammonia (2:2:1) and n-butanol/acetic acid/water (4:1:1) as the first- and second-dimension eluents, respectively, in analytical and semipreparative scales, was set up and verified by reversed-phase high-performance liquid chromatography (RP-HPLC) to be feasible and efficient to separate the hydrolysates. Five new DPP-IV-inhibitory peptides, four relatively large oligopeptides (MHQPPQPL, SPTVMFPPQSVL, VMFPPQSVL, and INNQFLPYPY), and AWPQYL were identified, and INNQFLPYPY showed a notable IC50 value of 40.08 μM as an uncompetitive inhibitor. Interactive effects on DPP-IV inhibition were also observed among separated fractions and pure synthetic peptide mixtures with concentration-dependent activity. The study gives new insights into goat casein hydrolysates with identified DPP-IV-inhibitory peptides efficiently isolated by 2D-TLC, which provides a simple and cost-efficient separation process and is compatible with liquid chromatography-tandem mass spectrometry ...Continue Reading

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Citations

Oct 21, 2015·Letters in Applied Microbiology·Z ZengS W Chen
Jan 14, 2016·Journal of Agricultural and Food Chemistry·Ying ZhangShangwu Chen
Apr 27, 2017·Foods·Eric Banan-Mwine DaliriByong H Lee
Apr 28, 2020·Antioxidants & Redox Signaling·Mathilde Foisy SauvéEmile Levy
Sep 18, 2020·Molecular Nutrition & Food Research·Shaun J SharkeyFinbarr P M O'Harte
Jan 27, 2019·International Journal of Molecular Sciences·Rui LiuHao Wu
Mar 11, 2018·Analytical and Bioanalytical Chemistry·Miriam Moreno-MontoroBeatriz Miralles
Jul 30, 2019·Journal of Food Biochemistry·Alice B Nongonierma, Richard J FitzGerald
Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing GaoXueying Mao
Jun 7, 2020·Applied Biochemistry and Biotechnology·Andréia Monique LermenDaniel Joner Daroit
Dec 16, 2020·Critical Reviews in Food Science and Nutrition·Caleb AcquahDominic Agyei
Jan 30, 2021·Food Research International·Talita C E S NascimentoKeila Aparecida Moreira
Dec 4, 2020·European Journal of Pharmacology·Bilal ÇakırTugba Tunali-Akbay
Mar 12, 2020·Journal of Agricultural and Food Chemistry·Fidel ToldráLeticia Mora
Aug 28, 2021·International Journal of Molecular Sciences·Priya Antony, Ranjit Vijayan

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