Isolation and identification of encapsulated strains of Bacteroides fragilis

The Journal of Infectious Diseases
D L KasperB F Polk

Abstract

One hundred three clinical isolates of Bacteroides fragilis were identified during a two-year period. Most of these isolates were strains of B. fragilis subspecies fragilis, which constitutes a minor component of the fecal flora in comparison with the other subspecies of B. fragilis. By use of several techniques for demonstration of capsules, it was found that only B. fragilis strains classified as subspecies fragilis were encapsulated. An indirect immunofluorescence assay was developed for identification of clinical isolates possessing capsular material that was immunologically similar to that found in the reference strain of B. fragilis subspecies fragilis. All strains examined that were classified as subspecies fragilis were positive in this assay for the capsular material, whereas strains of the other subspecies were negative. This tests represents a rapid and sensitive means of identifying the most prevalent anaerobic gram-negative bacillus involved in human infections. The capsular polysaccharide of B. fragilis subspecies fragilis is a unique factor associated with the predominant subspecies of B. fragilis isolated from clinical material.

Citations

Nov 1, 1982·Infection·S TabaqchaliD L Kasper
Mar 13, 1979·Medical Microbiology and Immunology·A A LindbergA Weintraub
May 1, 1987·Veterinary Immunology and Immunopathology·D L Emery
Aug 1, 1978·The Journal of Hygiene·K M Elhag, S Tabaqchali
Oct 7, 2006·Nature Reviews. Immunology·Sarkis K Mazmanian, D L Kasper
Apr 1, 1980·Antimicrobial Agents and Chemotherapy·S L Hansen
Jan 1, 1982·The Journal of Clinical Investigation·A B OnderdonkD L Kasper
Jan 19, 2012·Nutrients·Asa Hakansson, Goran Molin
Oct 1, 1984·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·A C Rodloff, H Hahn
Sep 13, 2005·Cellular Microbiology·Brian A Cobb, D L Kasper
May 31, 1988·American Journal of Surgery·D E Fry
Dec 1, 1981·The Journal of Applied Bacteriology·F Meisel-MikołajczykI Grzelak-Puczyńska
Dec 1, 1984·The Journal of Applied Bacteriology·F Meisel-MikołajczykJ Paelinck
May 17, 2006·Anaerobe·A B Onderdonk
Jan 1, 1987·Critical Reviews in Microbiology·I Brook
Mar 4, 2000·The Journal of Biological Chemistry·A O TzianabosD L Kasper
Feb 13, 2020·Biotechnology & Genetic Engineering Reviews·Thomas E MacCalmanStephen E Harding
Feb 1, 1988·The Journal of Applied Bacteriology·W C NobleD H Lloyd
Jan 1, 1990·Society for Applied Bacteriology Symposium Series·A Gilmour, J Harvey
Jan 1, 1990·Society for Applied Bacteriology Symposium Series·W C Noble
Jun 1, 1988·The Journal of Applied Bacteriology·K YoshidaS Takeuchi
Jan 1, 1985·The Journal of Applied Bacteriology·L A DevrieseG O Adegoke
Nov 1, 1987·The Journal of Applied Bacteriology·J Harvey, A Gilmour
Aug 1, 1980·The Journal of Applied Bacteriology·L A Devriese, V Hájek
Dec 1, 1992·Epidemiology and Infection·M A DeightonR Borland
Feb 1, 1993·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·H C Wegener
Aug 1, 1981·Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology·T Hofstad
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·W M Kalka-MollD L Kasper
Feb 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·A SchwanU Forsum
Jan 1, 1979·Medical Microbiology and Immunology·A WeintraubC E Nord
Dec 21, 2019·Journal of Molecular Biology·Ezequiel Valguarnera, Juliane Bubeck Wardenburg

Related Concepts

Antigens, Bacterial
Bacteroides fragilis
Cell Wall
Immunofluorescence Assay

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Related Papers

The Journal of Infectious Diseases
R G MathiasA R Ronald
Medical Microbiology and Immunology
A A LindbergA Weintraub
Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology
C HöhneG Hübner
© 2021 Meta ULC. All rights reserved