Abstract
Verots S3 cells derived from the African green monkey kidney were revealed to contain nine types of sulfoglycolipids by incorporating [35S]sulfate. These sulfated glycolipids were separated by DEAE-Sephadex column chromatography and preparative thin-layer chromatography (TLC). The major sulfoglycolipids were characterized using TLC, gas-liquid chromatography (GLC), mass spectrometry, solvolysis, TLC immunostaining, and nuclear magnetic resonance spectra as follows: V1, SM4s (GalCer I3-sulfate); V2, SM3 (LacCer II3-sulfate); V3, SM2a (Gg3Cer II3-sulfate); V4, globopentaosyl ceramide sulfate (Gb5Cer V3-sulfate); V5, (Gg4Cer II3-sulfate, IV3-NeuAc); V6, SB1a (Gg4Cer II3, IV3-bis-sulfate); and V8, (Gg4Cer II3-NeuAc, IV3-sulfate). Both V5 and V8 were sulfated gangliosides comprising both N-acetyl neuraminic acid and sulfate, and this was the first report on V8. A minor component V7 was identified as SM1a (Gg4Cer II3-sulfate) based on its behavior in TLC, GLC, and liquid secondary ion mass spectroscopy. It was postulated that this substance was a precursor of V6 (SB1a) and V5 (Gg4Cer II3-sulfate, IV3-NeuAc), and to date, its presence has not been demonstrated in nature. Another minor component V9 was identified as glucosyl ceramide s...Continue Reading
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Citations
Mar 2, 2011·Analytical and Bioanalytical Chemistry·Christian MarschingCarsten Hopf
Feb 17, 2010·Glycobiology·Yukio NiimuraKen-ichi Nagai
Apr 18, 2008·Journal of Virology·Tadanobu TakahashiTakashi Suzuki
Oct 7, 2014·Glycoconjugate Journal·Suchandra ChowdhuryChitra Mandal
Feb 18, 2016·Glycoconjugate Journal·Reiko AndoKoichi Furukawa
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Sep 25, 2015·Organic & Biomolecular Chemistry·Haruka HiroseMakoto Kiso
Feb 19, 2008·Analytical Chemistry·Ute DistlerJohannes Müthing