Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and hepatoprotective properties in tacrine-treated HepG2 cells

Journal of Agricultural and Food Chemistry
Min-Sup LeeHyeung-Rak Kim

Abstract

Four kinds of phlorotannins having antioxidant activity were isolated from the ethyl acetate fraction of Ecklonia stolonifera ethanolic extract. The structures of the phlorotannins were determined on the basis of spectroscopic evidence, including 1D and 2D nuclear magnetic resonance. The isolated phlorotannins showed potential radical-scavenging activities against 2,2-diphenyl-1-picrylhydrazyl and suppressed the intracellular reactive oxygen species in tacrine-treated HepG2 cells. Among them, eckol and 2-phloroeckol showed hepatoprotective activity in tacrine-treated HepG2 cells; however, phlorofucofuroeckol B and 6,6'-bieckol did not show the activity, even though having high antioxidant activity. Both eckol and 2-phloroeckol inhibited the expression of Fas-mediated cell-death proteins, including tBid, caspase-3, and poly(ADP-ribose) polymerase, and suppressed the release of cytochrome c from mitochondria to cytosol in a dose-dependent manner in tacrine-treated HepG2 cells. These results suggest that eckol and 2-phloroeckol are the principal hepatoprotective constituents of the ethyl acetate fraction of E. stolonifera ethanolic extract.

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Citations

Oct 4, 2012·BMC Complementary and Alternative Medicine·Eun-Ji JoungHyeung-Rak Kim
Oct 1, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Young-Jin JunHyeung-Rak Kim
Feb 25, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chunpeng GaoJun Cao
Nov 1, 2016·Biomolecules & Therapeutics·Chae-Young BangSe-Young Choung
Feb 2, 2017·International Journal of Molecular Sciences·Susete PinteusRui Pedrosa
Jan 24, 2019·Annual Review of Food Science and Technology·Charlotte JacobsenDitte B Hermund
Aug 26, 2017·Archives of Pharmacal Research·Prashamsa KoiralaJae Sue Choi
Jun 21, 2019·Marine Drugs·Bandana ManandharJae Sue Choi
Jul 5, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhi-Li ZhaoYi Liu
Sep 28, 2017·Marine Drugs·Mohammed Shariful AzamHyeung-Rak Kim
Oct 2, 2019·Experimental and Therapeutic Medicine·Mengya ZhangJun Wang
May 29, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammed Shariful AzamHyeung-Rak Kim
Nov 22, 2020·Marine Drugs·Lei CaoHyeung-Rak Kim

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