Isolation and identification of six Pneumocystis carinii genes utilizing codon bias

Gene
L D FletcherC C Dykstra

Abstract

Pneumocystis carinii pneumonia (PCP) is a leading cause of death among AIDS patients in the United States. Our analysis of P. carinii protein-coding genes has revealed a significant A + T codon bias. Polymerase chain reaction (PCR) was utilized to isolate and identify the genes encoding calmodulin, beta-tubulin, DNA polymerase II, and RNA polymerases I, II and III from P. carinii. Primer pairs were designed to incorporate P. carinii codon preference to known conserved protein regions from other organisms. This strategy should be useful for a large variety of P. carinii genes and assist in the comprehensive analysis of the genomic structure of this important pathogen.

References

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Related Concepts

X54209
PMCPOLII
TUBE1 gene
Genome
Pathogenic Organism
DNA-Directed RNA Polymerase
Pneumocystis Infections
SERRYG
Transcriptase
Calmodulin

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