Isolation and identification of steroidal saponins in Taiwanese yam cultivar (Dioscorea pseudojaponica Yamamoto)

Journal of Agricultural and Food Chemistry
Deng-Jye YangLucy Sun Hwang

Abstract

A new furostanol pentaoligoside and spirostanol tetraoligoside were isolated for the first time from yam tubers (Dioscorea pseudojaponica Yamamoto) from Taiwan, together with four known yam saponins, methyl protodioscin, methyl protogracillin, dioscin, and gracillin. Their structures were characterized as 26-O-beta-D-glucopyranosyl-22alpha-methoxyl-(25R)-furost-5-en-3beta,26-diol, 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-([alpha-L-rhamnopyranosyl-(1-->4)]-O-[alpha-L-rhamnopyranosyl-(1-->4)])-beta-D-glucopyranoside, and (25R)-spirost-5-en-3beta-ol 3-O-alpha-L-rhamnopyranosyl-(1-->2)-O-([alpha-L-rhamnopyranosyl-(1-->4)]-O-[alpha-L-rhamnopyranosyl-(1-->4)])-beta-D-glucopyranoside. The structural identification was performed using LC-MS and 1H and 13C NMR. The methanol extract of yam tubers was fractionated by XAD-2 column chromatography using a methanol/water gradient elution system to yield furostanol and spirostanol glycoside fractions. Preparative high-performance liquid chromatography, employing a C18 column and a mobile phase of methanol/water (69:31, v/v), was used to separate each furostanol glycoside, whereas a mobile phase of methanol/water (79:21, v/v) was used to resolve the individual spirostanol glycosides. The conversio...Continue Reading

Citations

Nov 21, 2014·Journal of Clinical Biochemistry and Nutrition·Toshiko Suzuki-YamamotoYoshitaka Takahashi
Jul 1, 2010·World Journal of Microbiology & Biotechnology·Wei LiuJinren Ni
Sep 2, 2011·Biological & Pharmaceutical Bulletin·Tong Ho KangSun Yeou Kim
Feb 19, 2010·Animal Science Journal = Nihon Chikusan Gakkaihō·Chuncheng XuKoji Toyokawa
Aug 25, 2004·Journal of Ethnopharmacology·S G SpargJ van Staden
May 16, 2006·Neurobiology of Disease·Jadwiga Turchan-CholewoAvindra Nath
Sep 20, 2016·Journal of Integrative Medicine·Musa Toyin Yakubu, Abdulquadri Funmbi Olutoye
Feb 18, 2010·Sensors and Actuators. B, Chemical·Nicholas StrandCristina E Davis
May 21, 2011·The American Journal of Chinese Medicine·Chuan-Sung ChiuWen-Huang Peng
Mar 17, 2017·Journal of Agricultural and Food Chemistry·Shun-Cheng TsengChiu-Liang Chen
Jan 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wen-Xia PiBao-Chang Cai
Oct 8, 2016·Organic & Biomolecular Chemistry·Peng WangMing Li
Sep 25, 2007·Magnetic Resonance in Chemistry : MRC·Patricia Y HayesJames J De Voss
Aug 12, 2005·Journal of the American College of Nutrition·Wen-Huey WuTzong-An Wang
Sep 13, 2019·BioMed Research International·Longfei YangLufei Wang

Related Concepts

furostanol I
High Pressure Liquid Chromatography Procedure
Glycosides
Hydrogen-Ion Concentration
Hydrolysis
In Vivo NMR Spectroscopy
Phytosterols
Saponin
Mass Spectrometry
Sterols

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