Isolation and identification of temperature tolerant phosphate solubilizing bacteria as a potential microbial fertilizer

World Journal of Microbiology & Biotechnology
Mohammad Reza SarikhaniRalf Greiner

Abstract

Isolation and identification of temperature tolerant phosphate solubilizing bacteria (TTPSB) and their use as microbial fertilizers was the main goal of the study. In this study, TTPSB were isolated from soil samples treated for 16 h at 55 °C. Their phosphate solubilizing activity was either evaluated in solid media by forming a clear zone (halo) or in liquid media by quantification of the soluble phosphate in the growth medium. Five colonies (RPS4, RPS6, RPS7, RPS8 and RPS9) were identified to be able to form a halo and two of the isolates (RPS9 and RPS7) tolerated a temperature of 55 °C. With tricalcium phosphate (TCP) as the sole P-source, the phosphate solubilizing capacity of RPS9 and RPS7 was determined to be 563.8 and 324.1 mg P L-1 in liquid Sperber medium, respectively. Both bacterial isolates were identified as Pantoea agglomerans by molecular and biochemical characterization. To be used as a microbial fertilizer a carrier system for the temperature tolerant bacteria consisting of rock phosphate, sulfur and bagasse was used. It could be established that the bacterial cell counts of the microbial fertilizers were acceptable for application after storage for 4 months at 28 °C. In a greenhouse experiment using pot cultur...Continue Reading

References

Jan 6, 2000·FEMS Microbiology Letters·C S NautiyalD Verma
Jan 27, 2004·International Journal of Systematic and Evolutionary Microbiology·Timothy G Lilburn, George M Garrity
Apr 24, 2013·Nucleic Acids Research·Grzegorz M BoratynIrena Zaretskaya
Jan 1, 2012·Scientifica·Bernard R Glick

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Citations

Dec 29, 2020·Frontiers in Plant Science·Igor Daniel Alves RibeiroLuciane Maria Pereira Passaglia
Feb 27, 2021·Extremophiles : Life Under Extreme Conditions·Averlane Vieira da SilvaAlysson Wagner Fernandes Duarte

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Datasets Mentioned

BETA
MK066125
MK070112

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
BLAST search
SPSS
MSTATC

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