PMID: 7940613Jun 1, 1994Paper

Isolation and identification of testosterone and androstenedione in the fungus Cochliobolus lunatus

Steroids
T Kastelic-SuhadolcD Zigon

Abstract

The cytosol of the filamentous fungus Cochliobolus lunatus was found to contain binding proteins for testosterone and androst-4-ene-3,17-dione. Both of these steroids were also found to be good exogenous substrates for the constitutive 17 beta-hydroxysteroid dehydrogenase, also found in this fungus. We were looking for the possible endogenous substrate. The procedures for isolation and identification of the endogenous steroid molecules in the fungus C. lunatus are described in this paper. The lipids were extracted from the cells and from the growth medium and purified by column and thin-layer chromatography. Analysis of the steroids, isolated from the cells of C. lunatus by gas chromatography and combination of gas chromatography-mass spectrometry, revealed the presence of testosterone and androst-4-ene-3,17-dione. Their structures were confirmed by comparison with the standards on the basis of chromatographic behavior and mass spectra. No such structures were found in the growth medium. Endogenous synthesis of androgens in this fungus was independently confirmed by the detection of radioactively labeled testosterone when the growing cells of C. lunatus were labeled with radioactive precursor molecule [5-3H] mevalonate.

References

Nov 15, 1992·FEMS Microbiology Letters·T LanisnikI Belic
Apr 1, 1992·Trends in Biochemical Sciences·J Lenard
Jan 1, 1990·General and Comparative Endocrinology·C SerniaW G Thomas
Mar 1, 1988·Journal of Steroid Biochemistry·A PlemenitasR Komel
Jul 6, 1988·Biochimica Et Biophysica Acta·M HirasawaD B Knaff
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D FeldmanD Harvey
Mar 15, 1993·FEMS Microbiology Letters·T Kastelic-Suhadolc, H Lenasi

Citations

Dec 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Zakelj-MavricI Belic
Jan 11, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tea Lanisnik Rizner, M Zakelj-Mavric
Oct 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·Tea Lanisnik RiznerM Zorko
Feb 24, 2009·Analytical Biochemistry·M TsivouC G Georgakopoulos
Feb 24, 2009·Analytical Biochemistry·M TsivouC G Georgakopoulos
Jul 7, 2005·Molecular and Cellular Endocrinology·Katja KristanTea Lanisnik Rizner
Jan 1, 1997·Mycoses·J Brasch
Sep 24, 2019·Steroids·Cristina Rauen RibasEdvaldo Antonio Ribeiro Rosa

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.

Related Papers

FEMS Microbiology Letters
T Kastelic-Suhadolc, H Lenasi
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
M Zakelj-MavricI Belic
FEMS Microbiology Letters
T LanisnikI Belic
© 2021 Meta ULC. All rights reserved