PMID: 7940613Jun 1, 1994Paper

Isolation and identification of testosterone and androstenedione in the fungus Cochliobolus lunatus

T Kastelic-SuhadolcD Zigon


The cytosol of the filamentous fungus Cochliobolus lunatus was found to contain binding proteins for testosterone and androst-4-ene-3,17-dione. Both of these steroids were also found to be good exogenous substrates for the constitutive 17 beta-hydroxysteroid dehydrogenase, also found in this fungus. We were looking for the possible endogenous substrate. The procedures for isolation and identification of the endogenous steroid molecules in the fungus C. lunatus are described in this paper. The lipids were extracted from the cells and from the growth medium and purified by column and thin-layer chromatography. Analysis of the steroids, isolated from the cells of C. lunatus by gas chromatography and combination of gas chromatography-mass spectrometry, revealed the presence of testosterone and androst-4-ene-3,17-dione. Their structures were confirmed by comparison with the standards on the basis of chromatographic behavior and mass spectra. No such structures were found in the growth medium. Endogenous synthesis of androgens in this fungus was independently confirmed by the detection of radioactively labeled testosterone when the growing cells of C. lunatus were labeled with radioactive precursor molecule [5-3H] mevalonate.


Nov 15, 1992·FEMS Microbiology Letters·T LanisnikI Belic
Apr 1, 1992·Trends in Biochemical Sciences·J Lenard
Jan 1, 1990·General and Comparative Endocrinology·C SerniaW G Thomas
Mar 1, 1988·Journal of Steroid Biochemistry·A PlemenitasR Komel
Jul 6, 1988·Biochimica Et Biophysica Acta·M HirasawaD B Knaff
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D FeldmanD Harvey
Mar 15, 1993·FEMS Microbiology Letters·T Kastelic-Suhadolc, H Lenasi


Dec 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Zakelj-MavricI Belic
Jan 11, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tea Lanisnik Rizner, M Zakelj-Mavric
Oct 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·Tea Lanisnik RiznerM Zorko
Feb 24, 2009·Analytical Biochemistry·M TsivouC G Georgakopoulos
Feb 24, 2009·Analytical Biochemistry·M TsivouC G Georgakopoulos
Jul 7, 2005·Molecular and Cellular Endocrinology·Katja KristanTea Lanisnik Rizner
Jan 1, 1997·Mycoses·J Brasch
Sep 24, 2019·Steroids·Cristina Rauen RibasEdvaldo Antonio Ribeiro Rosa

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