Mar 23, 2002

Isolation and identification of the major heparan sulfate proteoglycans in the developing bovine rib growth plate

The Journal of Biological Chemistry
Prasanthi GovindrajJohn R Hassell

Abstract

Heparan sulfate proteoglycans are thought to mediate the action of growth factors. The heparan sulfate-containing proteoglycans in extracts of the bovine fetal rib growth plate were detected using the monoclonal antibody 3G10, which recognizes a neoepitope generated by heparitinase digestion (David, G., Bai, X. M., Van der Schueren, B., Cassiman, J. J., and Van den Berghe, H. (1992) J. Cell Biol. 119, 961-975). The heparan sulfate proteoglycans that react with this antibody were identified using antisera to known proteoglycans; purified using CsCl density gradient centrifugation, molecular sieve, and ion exchange chromatography; and then characterized. The major heparan sulfate proteoglycans in the growth plate had core proteins of 200 kDa and larger and were identified as perlecan and aggrecan. These two heparan sulfate proteoglycans could be effectively separated from each other by CsCl density gradient centrifugation alone. Perlecan contained 25% heparan sulfate and 75% chondroitin sulfate. The heparan sulfate chains on growth plate perlecan were considerably smaller than the chondroitin sulfate chains, and the heparan sulfate disaccharide content was different than that found for heparan sulfate from either kidney, tumor ti...Continue Reading

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Mentioned in this Paper

Monoclonal Antibodies
Chloride Ion Level
Centrifugation, Density Gradient
Chondroitin Sulfate, Zinc Salt
Bone Structure of Rib
Tumor Tissue Sample
Proteoglycan
MYOZ2 gene
Antigenic Specificity
Tertiary Protein Structure

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