PMID: 9542613May 23, 1998Paper

Isolation and immunobiochemical characterization of a major allergen (65 kDa) from Fusarium equiseti

Allergy
J VermaS V Gangal

Abstract

Fusarium equiseti is one of the most important species in the class Deuteromycetes (Fungi Imperfecti). For proper diagnosis and immunotherapy, isolation and characterization of allergens of F. equiseti are necessary. In the present study, culture filtrate (CF) extract of F. equiseti was resolved into 35-37 bands on isoelectric focusing pI (3-9) and SDS-PAGE (mol. wt. 10-100 kDa). Most of them were glycoproteins, as identified by PAS staining. F. equiseti CF revealed 15 allergenic proteins on immunoblot with an allergic serum pool. It was fractionated into nine fractions (I-IX) on a Superose-12 column by FPLC. Fraction IV (65 kDa) and fraction VI (25 kDa) were found to be highly allergenic by IgE ELISA. A 65-kDa protein was observed as a major allergen because it was recognized by most of the patient sera on immunoblot. After elution from SDS-PAGE gel, it gave two bands of pI 7.4 and 6.0. Inhibition in IgE-binding components of F. equiseti CF with CF extracts of F. solani and F. moniliforme by immunoprint inhibition assay indicated the allergenicity shared between the extracts of Fusarium species. Data suggested that the 65-kDa is the major allergen in the Fusarium species and can be used for the treatment of allergic patients.

References

Jan 1, 1979·International Archives of Allergy and Applied Immunology·H M VijayI L Bernstein
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1988·Mycopathologia·J M FarberP M Scott
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Dec 1, 1973·Analytical Biochemistry·R A Kapitany, E J Zebrowski
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Feb 23, 1994·Molecular and Cellular Biochemistry·J VermaS V Gangal
Jan 1, 1994·International Archives of Allergy and Immunology·J Verma, S V Gangal
Nov 1, 1993·The Journal of Allergy and Clinical Immunology·A B CzupponX Baur

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Citations

Jun 26, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J VermaN Arora
Mar 28, 2012·Arhiv za higijenu rada i toksikologiju·Ali Reza KhosraviMohammad Hossein Yadegari
Mar 1, 2012·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Kiyofumi MochizukiKazuko Nishimura
Feb 26, 2009·FEMS Immunology and Medical Microbiology·Neetu KukrejaNaveen Arora

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