PMID: 9168469May 1, 1997Paper

Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe

Molecular Biology of the Cell
Abul Kalam AzadYasumi Ohshima

Abstract

Nucleocytoplasmic transport of mRNA is essential for eukaryotic gene expression. However, how mRNA is exported from the nucleus is mostly unknown. To elucidate the mechanisms of mRNA transport, we took a genetic approach to identify genes, the products of which play a role in that process. From about 1000 temperature -sensitive (ts- or cs-) mutants, we identified five ts- mutants that are defective in poly(A)+ RNA transport by using a situ hybridization with an oligo(dT)50 as a probe. These mutants accumulate poly(A)+ RNA in the nuclei when shifted to a nonpermissive temperature. All five mutations are tightly linked to the ts- growth defects, are recessive, and fall into four different groups designated as ptr 1-4 (poly(A)+ RNA transport). Interestingly, each group of mutants has a differential localization pattern of poly(A)+ RNA in the nuclei at the nonpermissive temperature, suggesting that they have defects at different steps of the mRNA transport pathway. Localization of a nucleoplasmin-green fluorescent protein fusion suggests that ptr2 and ptr3 have defects also in nuclear protein import. Among the isolated mutants, only ptr2 showed a defect in pre-mRNA splicing. We cloned the ptr2+ and ptr3+ genes and found that they e...Continue Reading

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Citations

Dec 19, 2007·Genes & Development·Natalie G FarnyPamela A Silver
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Carole GwizdekCatherine Dargemont
Sep 26, 2015·RNA Biology·Anna Domanska, Joanna Kaminska
Dec 24, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Eiji HiroseTakeharu Nishimoto
Aug 18, 2004·Progress in Neurobiology·Ashok N Hegde
Nov 21, 2007·Biochemical and Biophysical Research Communications·Taro MannenTokio Tani
Apr 20, 2004·Biochemical and Biophysical Research Communications·Tomoko AndohTokio Tani
Sep 27, 2003·Biochemical and Biophysical Research Communications·Abul Kalam AzadTokio Tani
Jan 14, 2012·Biochimica Et Biophysica Acta·Anna BabourFrançoise Stutz
Jan 14, 2012·Biochemical and Biophysical Research Communications·Nobuyoshi WatanabeTokio Tani
Dec 17, 2008·Current Protocols in Cell Biology·Kazuaki Tokunaga, Tokio Tani
Jul 15, 2003·Molecular Biology of the Cell·Marganit FaragoMordechai Choder
Feb 17, 2005·The Journal of Biological Chemistry·Carole GwizdekManuel S Rodriguez
Jan 11, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Fumitaka MizukiTokio Tani
Jan 29, 2000·Molecular and Cellular Biology·S Shinozaki-YabanaM Yamamoto
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Jan 29, 2013·Eukaryotic Cell·Rongpeng LiSheng Yuan
Jul 28, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·M WatanabeE Nishida
May 27, 2004·Journal of Cell Science·Takashi IdeueTokio Tani

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