Isolation and molecular characterization of Acanthamoeba genotypes in recreational and domestic water sources from Jamaica, West Indies

Journal of Water and Health
Cheridah D ToddJohn F Lindo

Abstract

Free living amoebae (FLA) are amphizoic protozoa that are ubiquitous in nature. Infection with FLA may result in neurological, ocular and skin infections. Exposure to Acanthamoeba occurs frequently through water contact and knowledge of the presence of the organisms in water sources is important in understanding transmission dynamics. The distribution of Acanthamoeba was studied in recreational and domestic water samples collected from across Jamaica. Morphological assessment and polymerase chain reaction revealed Acanthamoeba spp. isolates in 50.6% (42/83) and 17.3% (14/81) of recreational and domestic water, respectively. Sequencing of the DF3 region of the 18S rDNA resulted in the identification of genotypes T3, T4, T5, T10 and T11 corresponding to Acanthamoeba spp: A. griffini, A. triangularis, A. lenticulata, A. culbertsoni and A. hatchetti. Moreover, T4 was the most frequently isolated genotype in both recreational and domestic water. Thermotolerance and osmotolerance assays indicated that most isolates were potentially pathogenic. This is the first report of T3 and T10 genotypes in the Caribbean and the first report of these Acanthamoeba spp. in Jamaican waters. The study shows that there is potential risk of infection t...Continue Reading

References

Jul 1, 1992·The British Journal of Ophthalmology·D SealJ Dart
Aug 1, 1982·Applied and Environmental Microbiology·T J BrownE A Keys
Feb 1, 1981·The Journal of protozoology·F RiveraA M Lacy
Apr 9, 2001·American Journal of Ophthalmology·N A FroumisB J Glasgow
Apr 26, 2002·The British Journal of Ophthalmology·C F RadfordJ K G Dart
Apr 15, 2003·Clinical Microbiology Reviews·Francine Marciano-Cabral, Guy Cabral
Aug 18, 2004·International Journal for Parasitology·Frederick L Schuster, Govinda S Visvesvara
Apr 9, 2005·Journal of Clinical Microbiology·Gregory C BootonPaul A Fuerst
Nov 22, 2005·Annals of Tropical Medicine and Parasitology·J Lorenzo-MoralesA Ortega-Rivas
Apr 6, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Frederick L SchusterCarol A Glaser
Jun 16, 2006·FEMS Microbiology Reviews·Naveed Ahmed Khan
Apr 13, 2007·FEMS Immunology and Medical Microbiology·Govinda S VisvesvaraFrederick L Schuster
Nov 14, 2007·Journal of Water and Health·M E ShoffD V Seal
Aug 7, 2009·Interdisciplinary Perspectives on Infectious Diseases·Bruno da Rocha-AzevedoFrancine Marciano-Cabral
Dec 17, 2009·Parasitology Research·Wilawan PumidonmingJulia Walochnik
Dec 29, 2009·Experimental Parasitology·Christian GianinazziNorbert Müller
Jan 5, 2011·Environmental Science & Technology·Jacqueline M Thomas, Nicholas J Ashbolt
Feb 5, 2011·Emerging Infectious Diseases·Sadia ShakoorAfia Zafar
Nov 19, 2011·The Journal of Eukaryotic Microbiology·Berbeli AstorgaBasilio Valladares
Jan 11, 2012·Parasites & Vectors·Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Nov 21, 2012·Journal of Water and Health·Rahmat SolgiEhsan Nazemalhosseini Mojarad
Jul 25, 2013·The Journal of Eukaryotic Microbiology·Yvonne QvarnstromGovinda S Visvesvara
Nov 10, 2013·Water Research·Vincent DelafontYann Héchard
Jan 23, 2014·Parasitology Research·María Reyes-BatlleJacob Lorenzo-Morales
May 2, 2014·Parasitology Research·Alfonso Martín Cabello-VílchezJacob Lorenzo-Morales

❮ Previous
Next ❯

Citations

Jan 19, 2016·Parasitology Research·Thani ThammaratanaUNKNOWN Neglected, zoonosis and vector-borne disease research group
Oct 28, 2016·Experimental Parasitology·Federica Del ChiericoLorenza Putignani
Feb 28, 2018·Journal of Bacteriology·Tiffany A Claeys, Richard T Robinson
Oct 17, 2017·Acta Parasitologica·Ali HanilooElnaz Kadkhodamohammadi
Jul 3, 2019·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Somayeh MaghsoodlooradRahmat Solgi
Feb 14, 2019·Journal of Water and Health·Shobana GabrielRuqaiyyah Siddiqui

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.