Isolation and molecular cloning of a major wheat allergen, Tri a Bd 27K

Bioscience, Biotechnology, and Biochemistry
M KimotoH Tsuji

Abstract

Tri a Bd 27K is the predominant allergen in wheat. In the present study, this allergen was purified to homogeneity from wheat flour. The N-terminal amino acid sequences of the purified allergen and the peptides obtained by its digestion, with trypsin were determined, and the allergen was shown to be a glycoprotein with an Asn-linked sugar moiety containing fucose residues. A cDNA encoding the allergen was obtained by polymerase chain reaction (PCR). The cDNA codes for a protein of 203 amino acid residues, with a molecular mass of 22,803 Da, that has two tentative sites glycosylated at Asn residues. Homology analysis suggested that the allergen might belong to a family of gamma-interferon-inducible thiol reductases. The cDNA was expressed as a fusion protein with glutathione S-transferase in Escherichia coli. However, unlike the allergen purified from wheat, recombinant Tri a Bd 27K was not immunoblotted with IgE antibodies in the serum of a wheat-sensitive patient.

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Citations

Jan 9, 2010·Bioscience, Biotechnology, and Biochemistry·Miki HiemoriHideaki Tsuji
Feb 19, 2011·Expert Review of Proteomics·Gianfranco MamonePasquale Ferranti
Mar 24, 2010·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Maili LehtoAntti Lauerma
Jul 6, 2010·Contact Dermatitis·Hiroaki MatsuoEishin Morita
Oct 5, 2015·Allergology International : Official Journal of the Japanese Society of Allergology·Hiroaki MatsuoTakanori Taogoshi
Jan 13, 2015·The Journal of Allergy and Clinical Immunology·Ingrid SanderMonika Raulf
Apr 1, 2015·Journal of Agricultural and Food Chemistry·Melania Collado-RomeroPilar Prieto
Oct 12, 2011·International Archives of Allergy and Immunology·Sabine KespohlMonika Raulf-Heimsoth
Nov 2, 2013·Allergy, Asthma & Immunology Research·Santiago Quirce, Araceli Diaz-Perales
Jan 22, 2021·Journal of the Science of Food and Agriculture·Fengyun Ma, Byung-Kee Baik

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