PMID: 2110148May 1, 1990Paper

Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2

Journal of Bacteriology
K ShazandT Leighton

Abstract

Western blot (immunoblot) analysis of Bacillus subtilis cell extracts detected two proteins that cross-reacted with monospecific polyclonal antibody raised against Escherichia coli initiation factor 2 alpha (IF2 alpha). Subsequent Southern blot analysis of B. subtilis genomic DNA identified a 1.3-kilobase (kb) HindIII fragment which cross-hybridized with both E. coli and Bacillus stearothermophilus IF2 gene probes. This DNA was cloned from a size-selected B. subtilis plasmid library. The cloned HindIII fragment, which was shown by DNA sequence analysis to encode the N-terminal half of the B. subtilis IF2 protein and 0.2 kb of upstream flanking sequence, was utilized as a homologous probe to clone an overlapping 2.76-kb ClaI chromosomal fragment containing the entire IF2 structural gene. The HindIII fragment was also used as a probe to obtain overlapping clones from a lambda gt11 library which contained additional upstream and downstream flanking sequences. Sequence comparisons between the B. subtilis IF2 gene and the other bacterial homologs from E. coli, B. stearothermophilus, and Streptococcus faecium displayed extensive nucleic acid and protein sequence homologies. The B. subtilis infB gene encodes two proteins, IF2 alpha (7...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 28, 1977·Molecular & General Genetics : MGG·M SpringerM Grunberg-Manago
Feb 1, 1979·Molecular & General Genetics : MGG·M SpringerM Grunberg-Manago
Jan 18, 1977·Molecular & General Genetics : MGG·D J TipperH G Wittmann
Apr 1, 1977·Applied and Environmental Microbiology·R A DedonderF Kunst
Mar 30, 1976·Molecular & General Genetics : MGG·E LoveN C Brown
Dec 1, 1989·Microbiological Reviews·J B StockA M Stock
May 10, 1985·Nucleic Acids Research·J A PlumbridgeM Grunberg-Manago
Jun 1, 1985·Microbiological Reviews·P J Piggot, J A Hoch
Jul 10, 1987·Nucleic Acids Research·J F SandsJ W Hershey
Jan 1, 1988·Annual Review of Biochemistry·L Gold
Dec 1, 1987·Molecular & General Genetics : MGG·S MartinezS A Lacks
Jan 10, 1986·Nucleic Acids Research·E Sobel, H M Martinez
Jul 15, 1974·Journal of Molecular Biology·T Leighton
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·B Low
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C SacerdotM Grunberg-Manago
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J A PlumbridgeM Grunberg-Manago
Jan 1, 1982·Current Topics in Microbiology and Immunology·J Kreft, C Hughes
Jan 1, 1981·Molecular & General Genetics : MGG·C PortierM Grumberg-Manago
Jan 1, 1983·Molecular & General Genetics : MGG·T Kurihara, Y Nakamura
Jan 1, 1981·Molecular & General Genetics : MGG·R A Sharrock, T Leighton
Aug 23, 1982·FEBS Letters·N J CoppardB F Clark
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

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Citations

Nov 1, 1991·European Journal of Biochemistry·A VambutasA Tzagoloff
Jan 27, 1995·The Journal of Biological Chemistry·L Ma, L L Spremulli
Jan 15, 1997·European Journal of Biochemistry·H P VornlocherM Sprinzl
Feb 1, 1997·Genome Génome / Conseil National De Recherches Canada·R K StormsH Bussey
Jul 14, 2018·Microbiology Spectrum·Sezen MeydanAlexander S Mankin
Dec 1, 1991·Biochimie·S LaalamiM Grunberg-Manago
Dec 31, 1991·Biochemical and Biophysical Research Communications·N R NyengaardH U Sperling-Petersen
Jul 20, 1991·Journal of Molecular Biology·S LaalamiM Grunberg-Manago

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