Isolation and molecular identification of a strain belonging to the new species Zalaria obscura from a deteriorated wooden artwork.

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Luigia SabatiniRaffaella Campana

Abstract

We report the case of an outdoor deteriorated wooden sculpture of Madonna, completely blackened in the face, and thus suspected of fungal attack. A multi-disciplinary approach, including microbiological analysis, molecular biology, and Fourier transform infrared (FT-IR) spectroscopy, was applied to understand the real nature of the observed alteration. FT-IR showed that the blackening was due to the application of a natural terpene resin subjected to alteration over time. The microbiological assay allowed to isolate a particular black fungus that has been recovered in the vegetative phase, growing as the only species adapted to the examined substrate. Basic Local Alignment Search Tool (BLAST) analysis of the ITS (internal transcribed spacer) region sequence identified the fungus (LS31012019) as Zalaria obscura, a black yeast belonging to the new genus Zalaria, family Dothideales. Overall, this study evidenced the importance of a multi-disciplinary approach to understand the real causes of observed deterioration of artworks. More interestingly, the recovery of a strain identified as Z. obscura from this type of substrate is never reported in the literature and this finding could offer the possibility to investigate the role of t...Continue Reading

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Feb 24, 1999·International Journal of Systematic Bacteriology·B Esteve-ZarzosoA Querol
Dec 17, 2011·Applied Microbiology and Biotechnology·Helene C Eisenman, Arturo Casadevall
Jan 11, 2014·Microbial Ecology·Rodrigo OrtizRobert A Blanchette
Jan 7, 2016·Frontiers in Microbiology·Joshua D NosanchukArturo Casadevall
Aug 25, 2016·International Journal of Molecular Sciences·Anna KozirógBeata Gutarowska
Jul 22, 2018·Letters in Applied Microbiology·D BoniekM A de Resende Stoianoff

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Citations

Aug 21, 2021·Journal of Applied Microbiology·Jun YoshikawaKenji Maehashi

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Datasets Mentioned

BETA
MN480547

Methods Mentioned

BETA
infrared spectroscopy
PCR
electrophoresis

Software Mentioned

BLAST
BLASTN search

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