Isolation and molecular identification of wild Newcastle disease virus isolated from broiler farms of Diyala Province, Iraq

Veterinary World
Amer Khazaal Alazawy, Karim Sadun Al Ajeeli

Abstract

Newcastle disease virus (NDV) remains a major viral disease of poultry. The morbidity and mortality rates of chickens vaccinated with NDV in broiler farms in Diyala Province were 100% and 80%, respectively, rates due to suspected infection with the highly virulent NDV. The present study aimed to isolate and identify the NDV virus and evaluate its pathogenicity in infected broiler chickens at poultry farms. Broiler chickens at two commercial poultry farms were suspected of being infected with virulent NDV due to high mortality rates. Virus isolated from samples of intestinal tissues, lungs, trachea, spleen, kidneys, and air sacs was adapted in the allantoic cavity of embryonated specific-pathogen-free (SPF) chicken eggs. The NDV pathotype was determined based on the mean death time (MDT) in eggs as well as the intracerebral pathogenicity index (ICPI) and intravenous pathogenicity index pathogenicity indexes of the isolated samples. Broilers were experimentally infected by inoculation with fluids collected from the allantoic cavities of 60 broilers aged 35 days. Serological and molecular tests were followed by enzyme-linked immunosorbent assay to determine levels of anti-NDV immunoglobulin G, and isolates were identified using a ...Continue Reading

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Citations

Jul 15, 2020·Disaster Medicine and Public Health Preparedness·Deldar Morad AbdulahSherzad Khudeida Suleman

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
reverse-transcription PCR
Assay

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