Isolation and morphological and metabolic characterization of common endophytes in annually burned tallgrass prairie

Mycologia
Keerthi MandyamAri Jumpponen

Abstract

Dark septate endophytes (DSE) are common and abundant root-colonizing fungi in the native tallgrass prairie. To characterize DSE fungi were isolated from roots of mixed tallgrass prairie plant communities. Isolates were grouped according to morphology, and the grouping was refined by ITS-RFLP and/or sequencing of the ITS region. Sporulating species of Periconia, Fusarium, Microdochium and Aspergillus were isolated along with many sterile fungi. Leek resynthesis was used to quickly screen for DSE fungi among the isolates. Periconia macro-spinosa and Microdochium sp. formed typical DSE structures in the roots; Periconia produced melanized intracellular microsclerotia in host root cortex, whereas Microdochium produced abundant melanized inter- and intracellular chlamydospores. To further validate the results of the leek resynthesis growth responses of leek and a dominant prairie grass, Andropogon gerardii, were assessed in a laboratory resynthesis system. Leek growth mainly was unresponsive to the inoculation with Periconia or Microdochium, whereas Andropogon tended to respond positively. Select Periconia and Microdochium isolates were tested further for their enzymatic capabilities and for ability to use organic and inorganic nit...Continue Reading

References

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Citations

Sep 18, 2012·Mycorrhiza·Michael S MayerhoferKaren A Harper
Jul 11, 2013·BioMed Research International·Constantinos MoulasDimitrios G Karpouzas
Jan 30, 2015·Frontiers in Microbiology·Keerthi G Mandyam, Ari Jumpponen
Apr 30, 2016·Genome Announcements·Aaron S DavidGeorgiana May
Sep 9, 2016·FEMS Microbiology Ecology·Dániel G Knapp, Gábor M Kovács
Oct 26, 2014·Mycologia·Michael Stefan MayerhoferGavin Kernaghan Kernaghan
Jan 10, 2018·Frontiers in Microbiology·Carlos VergaraJerri E Zilli
Jan 31, 2019·The New Phytologist·Anais GibertMark Westoby
May 16, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Carlos VergaraJerri Édson Zilli
Nov 24, 2016·Ecology and Evolution·Carlos A Aguilar-Trigueros, Matthias C Rillig
Jul 24, 2020·Extremophiles : Life Under Extreme Conditions·Rómulo Oses-PedrazaMarco A Molina-Montenegro
Nov 30, 2019·Frontiers in Microbiology·Dániel G KnappGábor M Kovács
Apr 20, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Leila ShadmaniAkram Fatemi
Jul 27, 2021·Frontiers in Plant Science·Eric C PereiraIñigo Zabalgogeazcoa

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