PMID: 12757953May 22, 2003Paper

Isolation and mutation site determination of the temperature-sensitive murB mutants of Staphylococcus aureus

FEMS Microbiology Letters
Miki MatsuoKazuhisa Sekimizu

Abstract

The murB gene encodes UDP-N-acetylenolpyruvylglucosamine reductase and functions in bacterial peptidoglycan biosynthesis. A plasmid carrying the murB gene restored the temperature-sensitive growth of six Staphylococcus aureus mutants, in which peptidoglycan biosynthesis stopped at a restrictive temperature. Specific activity of UDP-N-acetylenolpyruvylglucosamine reductase in extracts from the mutants was lower than that from wild-type cells. Nucleotide sequence determination revealed that each mutant had a single amino acid substitution in the murB gene and five of six mutations were located within domain 3, where the proposed substrate binding site is located. These results suggest that the murB gene is essential for growth of S. aureus and that domain 3 is important for the MurB activity.

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May 23, 2002·FEMS Microbiology Letters·Chikara KaitoKazuhisa Sekimizu
Dec 21, 2002·Molecular Microbiology·Ahmed El ZoeibyRoger C Levesque

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Citations

Apr 27, 2012·The ISME Journal·Eamonn P CulliganColin Hill
Sep 23, 2011·The Journal of Biological Chemistry·Yuichi HanadaChikara Kaito
Feb 7, 2007·Infection and Immunity·Yasuhiko MatsumotoKazuhisa Sekimizu
Mar 11, 2016·Scientific Reports·Yasuhiko MatsumotoKazuhisa Sekimizu
Nov 8, 2006·Molecular Microbiology·Chikara KaitoKazuhisa Sekimizu
May 17, 2005·Drug Discovery Today·Jorge García-LaraSimon J Foster
Dec 9, 2014·Nature Chemical Biology·Hiroshi HamamotoKazuhisa Sekimizu
Jul 5, 2007·FEMS Microbiology Letters·Mihoko IshibashiKazuhisa Sekimizu
May 22, 2009·FEMS Microbiology Letters·Stijn van der VeenMarjon H J Wells-Bennik
Feb 5, 2014·The Journal of Biological Chemistry·Shunsuke NumataChikara Kaito
Aug 18, 2012·Molecular BioSystems·Haihong HaoZonghui Yuan

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