PMID: 6161143Jan 1, 1981Paper

Isolation and partial characterization of a major peanut allergen

The Journal of Allergy and Clinical Immunology
M I SachsJ W Yunginger

Abstract

We isolated and partially characterized a major peanut allergen (Peanut-I) using the radioallergosorbent test (RAST) to monitor the allergenicity of various fractions. Raw peanuts were pulverized, defatted, and extracted; the resulting crude extract (CPE) was fractionated by diethylaminoethyl (DEAE) cellulose anion-exchange chromatography using a linear salt gradient. Peak allergenic fractions were further purified by a second DEAE cellulose chromatography step, followed by a preparative polyacrylamide gel electrophoresis (PAGE) step. The final product, Peanut-I, contained 11% nitrogen and 8.7% carbohydrate and was homogeneous by both analytical PAGE and immunoelectrophoresis against rabbit anti-CPE. Peanut-I was heterogeneous by thin-layer electrofocusing and by PAGE in 1% sodium dodecyl sulfate. Biologic activity of Peanut-I was demonstrated by positive skin tests and leukocyte histamine release assays in patients with peanut allergy. By RAST inhibition assay Peanut-I did not account for all of the allergenic activity of CPE.

Citations

Jul 1, 1985·Clinical Reviews in Allergy·D D Metcalfe
Jun 1, 1984·The Journal of Allergy and Clinical Immunology·D D Metcalfe
Jan 1, 1996·Critical Reviews in Food Science and Nutrition·R K Bush, S L Hefle
Jan 1, 1996·Critical Reviews in Food Science and Nutrition·D D MetcalfeR L Fuchs
Jun 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C Loza, J Brostoff
Jun 11, 2005·International Archives of Allergy and Immunology·Richard E GoodmanRonald van Ree
Feb 1, 1995·The Journal of Allergy and Clinical Immunology·A W BurksR M Helm
Jun 1, 1990·The Journal of Allergy and Clinical Immunology·S B LehrerJ E Morgan
Nov 1, 1986·The Journal of Allergy and Clinical Immunology·D A Moneret-Vautrin
Jul 1, 1993·Current Problems in Pediatrics·A W Burks, H Sampson
Jul 1, 1990·The Journal of Allergy and Clinical Immunology·M U KeatingJ W Yunginger
Nov 1, 1996·The Journal of Allergy and Clinical Immunology·P A EigenmannH A Sampson
Nov 1, 1992·The Journal of Pediatrics·M Martín Esteban
Sep 1, 1991·The Journal of Allergy and Clinical Immunology·J T Chiu, I B Haydik
Nov 18, 2003·Allergy·H BernardP Scheinmann
Jan 1, 1985·Clinical Allergy·A S KempJ Simpson
Jul 24, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E C de JongA H Penninks
Nov 21, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M C ClarkeT P Dean
Aug 28, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A OlszewskiJ L Guéant
Jul 5, 2005·Molecular Nutrition & Food Research·Sander R PiersmaStef J Koppelman
Feb 13, 1999·The Journal of Biological Chemistry·S J KoppelmanH H de Jongh
Apr 1, 1996·Nutrition Reviews·J P Lallès, G Peltre
Aug 29, 1998·Allergy·W BurksG A Bannon
Jun 1, 1993·Zeitschrift für Ernährungswissenschaft·L UhlemannM Schlaak
Dec 5, 2002·Allergologia et immunopathologia·M J PereiraM Martín Esteban
Oct 24, 2007·Journal of Agricultural and Food Chemistry·H BernardJ M Wal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Allergy and Clinical Immunology
D BarnettM E Howden
The Journal of Allergy and Clinical Immunology
J A NordleeJ W Yunginger
The Journal of Allergy and Clinical Immunology
S L TaylorJ W Yunginger
The Journal of Allergy and Clinical Immunology
S A BockC D May
© 2021 Meta ULC. All rights reserved