Isolation and partial characterization of a protease involved in Fas-induced apoptosis

FEBS Letters
J SchlegelS Orrenius

Abstract

Protease involvement has been implicated in the signalling process of activation-induced apoptosis. Here we report the isolation of a protease from Jurkat T cells undergoing Fas-induced apoptosis. Although the protease probably is a serine protease, it seems to be distantly related to members of the ICE/ced-3/Ich-1(nedd-2) family. In a cell-free system using isolated thymocyte nuclei, the protease rapidly induces DNA fragmentation and morphological changes typically seen in apoptosis. Our results clearly show protease activation downstream to Fas-ligation and implicate an important role for the isolated protease in signalling of Fas-induced apoptosis.

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Citations

Jan 1, 1997·Journal of Cellular Biochemistry·B ZhivotovskyS Orrenius
Oct 1, 1996·Molecular and Cellular Biochemistry·H J Brömme, J Holtz
Oct 31, 1996·Experientia·B ZhivotovskyS Orrenius
Aug 1, 1996·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D VinatierD Subtil
Mar 1, 1996·DNA and Cell Biology·J YooC W Beattie
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K SamejimaW C Earnshaw
Feb 20, 2010·Cellular and Molecular Life Sciences : CMLS·Karen SchraderChristoph Borner
May 13, 2006·Biochemical and Biophysical Research Communications·A R O'ConnellC Stenson-Cox
Sep 25, 2007·Biochimica Et Biophysica Acta·A R O'Connell, C Stenson-Cox
Nov 29, 1996·The Journal of Biological Chemistry·B A VanceD M Segal
May 9, 2000·Cell Death and Differentiation·N Nakamura, Y Wada
Jan 26, 1996·The Journal of Biological Chemistry·J SchlegelD W Nicholson
Jan 1, 1997·Molecular and Cellular Biology·J M LenczowskiJ D Ashwell
Oct 29, 1997·Molecular and Cellular Biology·S KagayaY Kuchino

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis