PMID: 42385Sep 1, 1979

Isolation and partial characterization of three acidic proteinases in erythrocyte membranes

The Biochemical Journal
S PontremoliA De Flora

Abstract

1. The distribution of proteolytic activity in membranes from human erythrocytes and from rabbit reticulocytes and erythrocytes was investigated, after removal of leucocytes and platelets from the cell suspensions. 2. All membrane preparations displayed proteolytic activity in the acidic pH region only. Membranes from human and rabbit mature erythrocytes showed latent activity, which could be increased when extracted with a number of detergents. 3. Three active fractions were resolved either by gel chromatography of solubilized membrane extracts or by standard polyacrylamide-gel electrophoresis. The three proteinase activities (designated proteinases I, II and III) were purified from solubilized extracts of human erythrocyte membranes. 4. The relevant mol.wts. were around 80000, 40000 and 30000, respectively, and each of the three proteinases appeared to be composed of a single polypeptide chain. 5. Distinctive pH optima (in the range pH2.8-3.9) and different saturation profiles with globin as substrate were observed for proteinases I, II and III. 6. Dithioerythritol, Hg(2+) and Cu(2+) inhibited each of the three human enzymes, but more selective inhibitory effects were exerted by other modifiers of proteolytic enzymes and by h...Continue Reading

References

Jul 1, 1988·Toxicology and Applied Pharmacology·M A Runge-MorrisR F Novak
Aug 1, 1981·British Journal of Haematology·V Bocci
Jan 1, 1986·Journal of Free Radicals in Biology & Medicine·K J Davies
Oct 15, 1982·Archives of Biochemistry and Biophysics·E MelloniS Pontremoli
Jan 1, 1982·European Journal of Biochemistry·A M Golovtchenko-MatsumotoT Osawa
Jun 1, 1981·Biochemical and Biophysical Research Communications·D A Schafer, D E Hultquist
Jun 1, 1988·Biochemical Medicine and Metabolic Biology·K K Gambhir, S G Nerurkar
Jun 1, 1985·Archives of Biochemistry and Biophysics·U BenattiA De Flora
Sep 1, 1980·European Journal of Biochemistry·S PontremoliA De Flora
Aug 1, 1980·European Journal of Biochemistry·M MüllerS Rapoport

Related Concepts

Cations, Divalent
Exertion
Tissue Membrane
Mature Erythrocytes
Enzymes, antithrombotic
SDS-PAGE
Peptide Hydrolases
Hemin
Reticulocyte Count (Procedure)
Proteolytic enzymes for treatment of wounds and ulcers

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