Isolation and partial characterization of endothelial cell extracellular complexes

Journal of Cellular Physiology
V B HatcherP B Gordon

Abstract

Human endothelial cells release components into the growth medium that stimulate cell-substratum adhesion. Several macromolecular components were isolated by ultracentrifugation of the endothelial cell conditioned medium. The components were heterogeneous, consisting of several sizes when examined by sedimentation velocity and gel filtration. When the extracellular components were evaluated by electron microscopy, structurally discrete particles were observed. The extracellular components and the complexes mediated cell-substratum adhesion to both human umbilical and arterial endothelial cells. The majority of the extracellular components that promote endothelial cell adhesion were pelleted by ultracentrifugation. Although the complexes contained fibronectin, antibodies to fibronectin did not inhibit cell adhesion to the complexes. Significant inhibition of endothelial cell adhesion was observed in the presence of heparin and heparan sulfate. The supernatant fraction following ultracentrifugation of the growth medium contained a component that suppressed endothelial cell adhesion to culture dishes coated with fibronectin, type I collagen, and endothelial cell complexes. SDS-polyacrylamide gel electrophoresis indicated that the ...Continue Reading

References

Jul 1, 1976·The Journal of Experimental Medicine·E A JaffeR Nachman
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E J MacarakN A Kefalides
Jun 1, 1978·The Journal of Experimental Medicine·E A Jaffe, D F Mosher
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Jan 1, 1985·The Journal of Cell Biology·D Schubert, M LaCorbiere
Apr 1, 1983·The Journal of Cell Biology·D SchubertC Birdwell
Dec 27, 1983·Biochimica Et Biophysica Acta·D T WoodleyH K Kleinman
Oct 15, 1984·Biochemical and Biophysical Research Communications·G Conn, V B Hatcher
Sep 1, 1983·In Vitro·P B GordonV B Hatcher
Jul 1, 1983·Journal of Cellular Physiology·E J Macarak, P S Howard
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D Schubert, M LaCorbiere
Jan 1, 1982·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H Sage, P Bornstein
May 1, 1982·Experimental Cell Research·D K MacCallumS R Ledbetter
May 1, 1981·Journal of Cellular Physiology·D GospodarowiczN Savion
Nov 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H SageP Bornstein

❮ Previous
Next ❯

Citations

Apr 1, 1990·The Journal of Clinical Investigation·D C TompkinsF D Lowy
Jul 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J AndoE M Levine
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T N Wight

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.