Isolation and partial characterization of neurofibrillary tangles and amyloid plaque core in Alzheimer's disease: immunohistological studies

Journal of Neuropathology and Experimental Neurology
P D GorevicB Frangione

Abstract

Fractions enriched in neurofibrillary tangles (NFT) and amyloid fibrils were isolated from the cerebral cortex of three cases of senile dementia of the Alzheimer type. Distilled water suspensions of these fractions were excluded from all pore size gels and resisted digestion with various proteolytic enzymes. Formic acid/chloroform treatment of each fraction resulted in the appearance of 4,000-6,000, 15,000-17,000 and 24,000 molecular weight proteins, with concomitant diminution in the amount of excluded material at the top of each gel. The 4,000-6,000 dalton band was best seen in fractions containing randomly arranged amyloid fibrils, and its amino acid composition resembled that of the recently reported "beta" protein. A polyclonal antiserum to purified NFT reacted with tangles in neurons and in dystrophic neurites around plaques by immunoperoxidase staining. No reaction was obtained with cerebrovascular or plaque core amyloid immunohistologically, or with the 4-6 kD protein on immunoblots. Cross-reactivity with the neurofibrillary lesions occurring in Pick's disease, progressive supranuclear palsy, postencephalitic Parkinsonism and dementia pugilistica was also seen. Specific binding of this antiserum to the double filamentou...Continue Reading

Citations

Jan 1, 1987·Acta Neuropathologica·S A BobinH M Wisniewski
Oct 14, 2009·Journal of Neural Transmission·Oliver WirthsThomas A Bayer
Oct 1, 1992·Journal of the Neurological Sciences·N J CairnsP L Lantos
Sep 1, 1989·Neurobiology of Aging·A D Snow, T N Wight
Feb 29, 1988·Neuroscience Letters·D M MannE Torgerson
Jul 3, 1990·Neuroscience Letters·G GiacconeO Bugiani
Dec 12, 2001·Neurochemistry International·Dennis J Selkoe
Jan 24, 2002·Neurobiology of Aging·Mark A LovellWilliam R Markesbery
Jun 26, 2002·Journal of Clinical Pharmacy and Therapeutics·A F MaioriniR B Raffa
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S G van DuinenB Frangione
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D J SelkoeT Oltersdorf
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·J NäslundP Greengard
Oct 4, 2011·The Journal of Biological Chemistry·Sadim JawharThomas A Bayer
Jun 8, 2012·Cold Spring Harbor Perspectives in Medicine·Colin L Masters, Dennis J Selkoe
Jan 1, 1995·Acta Neurologica Scandinavica·C A MangoneR E Sica
Dec 4, 2010·Circulation Research·Susan A AustinZvonimir S Katusic
Jul 12, 2012·Journal of Geriatric Cardiology : JGC·Ashley C TorregrossaNathan S Bryan
Mar 10, 2016·Acta Neuropathologica Communications·Jochim ReinertOliver Wirths
Jan 1, 1989·Neuropathology and Applied Neurobiology·H Braak, E Braak
Aug 25, 2015·Frontiers in Microbiology·Christian W RemmeleMarcus T Dittrich
Apr 1, 2014·Oxidative Medicine and Cellular Longevity·Paul Carrillo-MoraLaura Colín-Barenque
Jul 27, 2011·Biochemical Society Transactions·Thomas A Bayer, Oliver Wirths
Dec 15, 1987·Biochemistry·F PrelliB Frangione
Jul 31, 2007·Neurologic Clinics·Christopher B Eckman, Elizabeth A Eckman
Jan 1, 1994·Critical Reviews in Clinical Laboratory Sciences·J D Sipe
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anyang SunGuoying Bing
Sep 10, 2014·Proteomics. Clinical Applications·Sarah PlumCaroline May
Nov 1, 1993·Journal of Neurochemistry·C Vigo-PelfreyD B Schenk
Mar 1, 1991·The Japanese Journal of Psychiatry and Neurology·S KatsuragiR Kuramoto
Apr 29, 2005·Journal of Neurochemistry·A L Brunkan, A M Goate

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.

Alzheimer's Disease: APP

Amyloid precursor protein (APP) proteolysis is critical for the development of Alzheimer's disease, a neurodegenerative disease associated with accumulation of amyloid plaques in the brain. Here is the latest research on APP and Alzheimer's disease.