Isolation and partial characterization of Escherichia coli mutants with altered glycyl transfer ribonucleic acid synthetases.

Journal of Bacteriology
W R Folk, P Berg

Abstract

Isolates with mutations in glyS, the structural gene for glycyl-transfer ribonucleic acid (tRNA) synthetase (GRS) in Escherichia coli, are frequently found among glycine auxotrophs. Extracts of glyS mutants have altered GRS activities. The mutants grow with normal growth rates in minimal media when high levels of glycine are provided. No other metabolite of a variety tested is capable of restoring normal growth. The glyS mutants fail to make ribonucleic acid (RNA) when depleted of exogenous glycine in strains which are RC(str) but do so when the cells are RC(rel). In contrast, biosynthetic mutants which are unable to synthesize glycine (glyA mutants) do not make RNA when deprived of glycine even if they are RC(rel); in this case, RNA is synthesized upon glycine deprivation only when the nucleic acid precursors made from glycine are provided in the medium. The level of serine transhydroxymethylase is unaltered in extracts of any of the glyS mutants, even though the level of charged tRNA(Gly) is at least 20-fold lower than that found in a prototrophic parent; this indicates that, if there is control over the synthesis of serine transhydroxymethylase, it is not modified by reduced levels of charging of the major species of tRNA(Gly).

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Citations

Jun 4, 2008·Annual Review of Biochemistry·Paul Berg
Dec 1, 2004·EcoSal Plus·George V Stauffer
Mar 1, 1976·Bacteriological Reviews·B J BachmannA L Taylor
Jun 1, 1971·Journal of Bacteriology·T Tosa, L I Pizer
Nov 1, 1971·Journal of Bacteriology·R R Russell, A J Pittard
Mar 1, 1981·Journal of Bacteriology·T T Chan, E B Newman
Jan 1, 1979·Journal of Bacteriology·J M GrüllA Böck
Dec 1, 1974·Journal of Bacteriology·G V StaufferJ E Brenchley
Dec 1, 1974·Journal of Bacteriology·S T Chung, G R Greenberg
Jun 1, 1975·Journal of Bacteriology·J Fraser, E B Newman
Jan 1, 1987·Infection and Immunity·F E AustinH H Winkler
Jun 14, 1971·Journal of Molecular Biology·W R Folk, P Berg

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