PMID: 991851Oct 1, 1976Paper

Isolation and partial characterization of elastase from dog granulocytes

European Journal of Biochemistry
M Delshammar, K Ohlsson


1. An elastolytic enzyme has been isolated from dog granulocyte leukocytes. The purification procedure included preparation of the granula fraction, chromatography on Sephadex G-75 and ion-exchange chromatography on SP-Sephadex C-50 at pH 6.0. 2. The elastase isolated was homogeneous in analytical disc electrophoresis and showed in sodium dodecylsulfate electrophoresis a single protein component with the molecular weight of 24800. The enzyme lacked tyrosine and lysine and the N-terminal amino acid was phenylalanine. No carbohydrate or sialic acid were detected. 3. The dog granulocyte elastase showed similar activities as human granulocyte elastase on elastin and fibrin. The Km value for 3-carboxypropionyl-L-alanyl-L-alanyl-L-alanyl-p-nitroanilide was 2.50 mM and the pH optimum 8.5. The elastase preparation obtained was 99.5% active as judged from active-site titration. 4. The enzyme is a cationic protein and shows pronounced trailing on agarose gel electrophoresis. 5. A monospecific antiserum against the purified enzyme was produced in rabbits.


Jan 1, 1976·Scandinavian Journal of Immunology·U JohnsonI Olsson
Aug 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K Ohlsson, A S Olsson
Feb 24, 1976·Biochemistry·R J Baugh, J Travis
Oct 1, 1971·Scandinavian Journal of Clinical and Laboratory Investigation·K Ohlsson
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·B G Johansson
Dec 1, 1974·Scandinavian Journal of Clinical and Laboratory Investigation·K Ohlsson, I Olsson
Mar 29, 1968·Science·G S LazarusH M Fullmer
Oct 1, 1971·Scandinavian Journal of Clinical and Laboratory Investigation·K Ohlsson
Sep 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·D W Edlow, W H Sheldon
Dec 1, 1960·The Journal of Experimental Medicine·Z A COHN, J G HIRSCH

❮ Previous
Next ❯


Aug 1, 1986·The Journal of Clinical Investigation·V V DamianoG Weinbaum
Dec 1, 1985·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·R GeigerM Jochum
Aug 15, 1984·Archives of Biochemistry and Biophysics·G OshimaM Yamada
Dec 1, 1986·The Journal of Surgical Research·R WinnJ Hildebrant
Jan 1, 1977·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H SchiesslerH Fritz
Jan 1, 1982·Scandinavian Journal of Plastic and Reconstructive Surgery·L Ekerot, K Ohlsson
Feb 1, 1990·Scandinavian Journal of Clinical and Laboratory Investigation·L AxelssonK Ohlsson

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.