PMID: 991851Oct 1, 1976Paper

Isolation and partial characterization of elastase from dog granulocytes

European Journal of Biochemistry
M Delshammar, K Ohlsson

Abstract

1. An elastolytic enzyme has been isolated from dog granulocyte leukocytes. The purification procedure included preparation of the granula fraction, chromatography on Sephadex G-75 and ion-exchange chromatography on SP-Sephadex C-50 at pH 6.0. 2. The elastase isolated was homogeneous in analytical disc electrophoresis and showed in sodium dodecylsulfate electrophoresis a single protein component with the molecular weight of 24800. The enzyme lacked tyrosine and lysine and the N-terminal amino acid was phenylalanine. No carbohydrate or sialic acid were detected. 3. The dog granulocyte elastase showed similar activities as human granulocyte elastase on elastin and fibrin. The Km value for 3-carboxypropionyl-L-alanyl-L-alanyl-L-alanyl-p-nitroanilide was 2.50 mM and the pH optimum 8.5. The elastase preparation obtained was 99.5% active as judged from active-site titration. 4. The enzyme is a cationic protein and shows pronounced trailing on agarose gel electrophoresis. 5. A monospecific antiserum against the purified enzyme was produced in rabbits.

References

Jan 1, 1976·Scandinavian Journal of Immunology·U JohnsonI Olsson
Aug 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K Ohlsson, A S Olsson
Feb 24, 1976·Biochemistry·R J Baugh, J Travis
Oct 1, 1971·Scandinavian Journal of Clinical and Laboratory Investigation·K Ohlsson
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·B G Johansson
Dec 1, 1974·Scandinavian Journal of Clinical and Laboratory Investigation·K Ohlsson, I Olsson
Mar 29, 1968·Science·G S LazarusH M Fullmer
Oct 1, 1971·Scandinavian Journal of Clinical and Laboratory Investigation·K Ohlsson
Sep 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·D W Edlow, W H Sheldon
Dec 1, 1960·The Journal of Experimental Medicine·Z A COHN, J G HIRSCH

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Citations

Aug 1, 1986·The Journal of Clinical Investigation·V V DamianoG Weinbaum
Dec 1, 1985·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·R GeigerM Jochum
Aug 15, 1984·Archives of Biochemistry and Biophysics·G OshimaM Yamada
Dec 1, 1986·The Journal of Surgical Research·R WinnJ Hildebrant
Jan 1, 1977·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H SchiesslerH Fritz
Jan 1, 1982·Scandinavian Journal of Plastic and Reconstructive Surgery·L Ekerot, K Ohlsson
Feb 1, 1990·Scandinavian Journal of Clinical and Laboratory Investigation·L AxelssonK Ohlsson

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