PMID: 6165999Jan 1, 1981Paper

Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer

Proceedings of the National Academy of Sciences of the United States of America
A K SoodS M Weissman

Abstract

We have isolated a cDNA clone for one of the HLA-B locus alloantigens by hybridization with a 30-nucleotide-long DNA probe. The probe was isolated from a reverse transcriptase (RNA-dependent DNA nucleotidyltransferase)-catalyzed cDNA synthesis reaction on poly(A)-mRNA in which an oligonucleotide (5'-32P)dC-T-T-C-T-C-C-A-C-A-TOH served as a primer and in which dideoxynucleoside triphosphates were used to reduce the size and heterogeneity of the cDNA products. The desired cDNA clone was isolated from a library of recombinant cDNA clones in the plasmid pBR322. The partial nucleotide sequence of the cDNA clone corresponds to the amino acid sequence of HLA-B7 antigen. The approach described in this paper is extremely sensitive and may be useful in cloning other genes for which the corresponding mRNA is present at low levels. This cDNA clone is nearly full length and can be used to isolate and to study the genes within the HLA region and to obtain expression of HLA-B peptides in cells.

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Citations

Nov 1, 1990·Journal of Cellular Biochemistry·G ZambettiG Stein
Jan 1, 1988·Human Genetics·M Bar-EliM J Cline
Jan 1, 1986·Immunogenetics·A H KottmannM H Roos
Jan 1, 1981·Immunogenetics·A A ReyesR B Wallace
Jan 1, 1986·Immunogenetics·B W DucemanF C Grumet
Jan 1, 1985·Immunogenetics·P ChardonM Vaiman
Jan 1, 1985·Immunogenetics·A K SoodS M Weissman
Jan 1, 1987·Immunogenetics·A J AlexanderJ G Woodward
May 1, 1984·Somatic Cell and Molecular Genetics·D N HowellP Cresswell
Mar 1, 1989·Clinical & Experimental Metastasis·F Ruiz-CabelloC Rivas
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Jan 1, 1987·Immunologic Research·B H KollerR DeMars
Jun 1, 1991·Aging : Clinical and Experimental Research·P E SlagboomJ Vijg
Jan 1, 1993·Advances in Enzyme Regulation·B S MitchellJ S Dayton
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Jan 1, 1988·Molecular Immunology·H L ChengH Kohler
Jan 15, 1992·Veterinary Immunology and Immunopathology·M A Miller-Edge, M B Worley
Apr 1, 1983·Human Immunology·R PayneL M Young
Jul 1, 1993·Human Immunology·S G RodriguezC K Hurley

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