PMID: 8599178Oct 1, 1995Paper

Isolation and properties of a blood coagulation factor X activator from the venom of king cobra (Ophiophagus hannah)

Toxicon : Official Journal of the International Society on Toxinology
W H LeeR Gao

Abstract

A specific blood coagulation factor X activator was purified from the venom of Ophiophagus hannah by gel filtration and two steps of FPLC Mono-Q column ion-exchange chromatography. It showed a single protein band both in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and alkaline polyacrylamide gel electrophoresis. The mol. wt was estimated to be 62,000 in non-reducing conditions and 64,500 in reducing conditions by SDS-PAGE. The isoelectric point was found to be pH 5.6. The enzyme had weak amidolytic activities toward CBS 65-25, but it showed no activities on S-2266, S-2302, thrombin substrate S-2238, plasmin substrate S-2251 or factor Xa substrate S-2222. It had no arginine esterase activity toward substrate benzoylarginine ethylester (BAEE). The enzyme activated factor X in vitro and the effect was absolutely Ca2+ dependent, with a Hill coefficient of 6.83. It could not activate prothrombin nor had any effect on fibrinogen and thus appeared to act specifically on factor X. The procoagulant activity of the enzyme was almost completely inhibited by serine protease inhibitors like PMSF, TPCK and soybean trypsin inhibitor; partially inhibited by L-cysteine. Metal chelator EDTA did not inhibit its procoagula...Continue Reading

References

Nov 26, 1976·Biochimica Et Biophysica Acta·Y FurukawaK Hayashi
Oct 29, 1991·Biochemistry·E W DavieW Kisiel
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·N H Tan, M N Saifuddin
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·Y Yamakawa, T Omori-Satoh
May 20, 1988·Cell·B Furie, B C Furie
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·M F el-AsmarA Tu
Nov 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·Z Y LiE C Lian
May 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·Y ZhangS W Zu

❮ Previous
Next ❯

Citations

Feb 27, 2001·Toxicon : Official Journal of the International Society on Toxinology·Q B LiT Kosugi
Jun 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Wen-Hui LeeYun Zhang
Oct 16, 2003·Toxicon : Official Journal of the International Society on Toxinology·I B SundellR D G Theakston
Dec 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·F S Markland
Aug 2, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mari SamelJüri Siigur
Nov 15, 2012·Journal of Proteome Research·Zi-Chao LiuYun Zhang
Jul 2, 2008·Toxicon : Official Journal of the International Society on Toxinology·Jianbo WuYuliang Xiong
Jun 5, 2007·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYun Zhang
Apr 6, 2007·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYun Zhang
Feb 3, 2005·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYu-Liang Xiong
Aug 11, 2006·Toxicon : Official Journal of the International Society on Toxinology·B L DhananjayaCletus J M D'Souza
Feb 22, 2011·Toxicon : Official Journal of the International Society on Toxinology·Weiwei LeiYun Zhang
Feb 1, 2011·Toxicon : Official Journal of the International Society on Toxinology·Tamara SajevicIgor Križaj
Sep 29, 2019·Toxins·Tarek Mohamed Abd El-AzizJames D Stockand
Sep 26, 2015·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Sami U Khan, Saad S Al-Saleh
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Ying-Ying HeYun Zhang
Dec 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Shu-Rong ZhongYun Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

Toxicon : Official Journal of the International Society on Toxinology
Y ZhangC Bon
Toxicon : Official Journal of the International Society on Toxinology
Y ZhangS W Zu
Toxicon : Official Journal of the International Society on Toxinology
N H Tan, M N Saifuddin
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Ying-Xia ZhangYun Zhang
© 2021 Meta ULC. All rights reserved