PMID: 7974840Jan 1, 1994

Isolation and properties of a protein with molecular mass of 50 kDa from free cytoplasmic mRNP-particles of rabbit reticulocytes

Ukrainskiĭ biokhimicheskiĭ zhurnal
V MynykhV M Evdokimova

Abstract

A method of purification and isolation of the major protein with molecular mass of 50 kDa from free cytoplasmic mRNP particles of rabbit reticulocytes is described. According to the SDS-gel electrophoresis data a degree of isolated protein purity in the preparation is 95%. An isoelectric point of 50 kDa protein is found to be within 9.5 units of pH. A great share of glycine (20%) is typical of p50 amino acid composition as well as for other proteins of mRNP-particles.

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