A restriction endonuclease was isolated from Bacillus stearothermophilus1503-4R (Bst1503) and purified to homogeneity. The enzyme required Mg2+ ion as a cofactor. Bst1503 exhibited maximal activity between pH 7.5 and 8.0, between 60 and 65 degrees C, and with about 0.2 mM Mg2+. Bst1503 was not inactivated after exposure at 55 or 65 degrees C for up to 10 h. After 2 h of incubation at 70 degrees C, Bst1503 was inactivated by 65%. Bst1503 was rapidly inactivated at 75 degrees C. A single protein-staining band having a molecular weight of 46,000 was observed when Bst1503 was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was found to exist in two active forms, the predominating form with an S value of 8.3 (180,000) and the second form with an S value of 5.4 (96,000). No conversion between the 8.3S and 5.4S forms was observed after storage. Bst1503 recognized six sites in TP-1C deoxyribonucleic acid (DNA), one site in pSC101 and simian virus 40 DNAs, and three sites in lambdavir DNA. Bst1503 and BamHI were determined to be isoschizomers. The effect of temperatures on the activity and stability of BamHI was determined.
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis
Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes
Host specificity of DNA produced by Escherichia coli, X. In vitro restriction of phage fd replicative form
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification
The isolation and properties of non-restricting mutants of two different host specificities associated with drug resistance factors
Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA
The effect of B specific restriction and modification of DNA on linkage relationships in f1 bacteriophage. I. Studies on the mechanism of B restriction in vivo
Isolation of a Bacillus stearothermophilus mutant exhibiting increased thermostability in its restriction endonuclease.
Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)
Bacteriophage: Phage Therapy
Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.