Isolation and properties of a thermostable restriction endonuclease (ENDO R-Bst1503).

Journal of Bacteriology
J F Catterall, N E Welker

Abstract

A restriction endonuclease was isolated from Bacillus stearothermophilus1503-4R (Bst1503) and purified to homogeneity. The enzyme required Mg2+ ion as a cofactor. Bst1503 exhibited maximal activity between pH 7.5 and 8.0, between 60 and 65 degrees C, and with about 0.2 mM Mg2+. Bst1503 was not inactivated after exposure at 55 or 65 degrees C for up to 10 h. After 2 h of incubation at 70 degrees C, Bst1503 was inactivated by 65%. Bst1503 was rapidly inactivated at 75 degrees C. A single protein-staining band having a molecular weight of 46,000 was observed when Bst1503 was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme was found to exist in two active forms, the predominating form with an S value of 8.3 (180,000) and the second form with an S value of 5.4 (96,000). No conversion between the 8.3S and 5.4S forms was observed after storage. Bst1503 recognized six sites in TP-1C deoxyribonucleic acid (DNA), one site in pSC101 and simian virus 40 DNAs, and three sites in lambdavir DNA. Bst1503 and BamHI were determined to be isoschizomers. The effect of temperatures on the activity and stability of BamHI was determined.

References

Jul 15, 1975·Journal of Molecular Biology·G F Vovis, N D Zinder
Sep 5, 1975·Journal of Molecular Biology·G A Wilson, F E Young
Dec 4, 1975·Nature·W PfeifferH G Zachau
Nov 1, 1972·Journal of Immunological Methods·J Eder
Jun 1, 1973·Journal of Bacteriology·R GromkovaS Goodgal
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·G R ReeckH A Sober
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S L Mowbray, A Landy
Jan 1, 1972·Annual Review of Biochemistry·M MeselsonJ Heywood
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A HabermanM Meselson
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·K Horiuchi, N D Zinder
Jan 1, 1974·Progress in Nucleic Acid Research and Molecular Biology·W Arber
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R T Simpson
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G F VovisN D Zinder
Nov 15, 1974·Journal of Molecular Biology·A Haberman
Apr 1, 1973·Journal of Virology·N D Lees, N E Welker
Mar 23, 1968·Nature·M Meselson, R Yuan
Apr 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S Linn, W Arber
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Nov 19, 1969·Biochimica Et Biophysica Acta·D Roulland-Dussoix, H W Boyer
Mar 14, 1972·Biochemistry·B L McConaughy, B J McCarthy
Mar 1, 1972·Archives of Biochemistry and Biophysics·D W Darnall, I M Klotz
Nov 7, 1969·Annals of the New York Academy of Sciences·D E Roark, D A YPHANTIS
Jul 28, 1970·Journal of Molecular Biology·H O Smith, K W Wilcox
Jul 1, 1969·Journal of Bacteriology·U N Streips, N E Welker
Mar 1, 1964·Biochemistry·D A YPHANTIS
Jan 1, 1965·Journal of Bacteriology·N E Welker, L L CAMPBELL

Citations

Sep 1, 1978·European Journal of Biochemistry·C KonczP Venetianer
Apr 20, 1987·FEBS Letters·P L ManachiniL Benazzi
Aug 1, 1979·Quarterly Reviews of Biophysics·P Modrich
Jul 1, 1981·European Journal of Biochemistry·R Imber, T A Bickle
Jan 1, 1982·CRC Critical Reviews in Biochemistry·P Modrich
Sep 14, 2010·Nucleic Acids Research·Eva Scheuring VanameeA K Aggarwal
Dec 1, 1983·Applied and Environmental Microbiology·A A YayanosA S Dietz
Mar 1, 1979·Journal of Bacteriology·D J ClantonR V Miller

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