Isolation and properties of extracellular alkaline phosphatase from Bacillus intermedius
Oct 1, 1998
Margarita Rashidovna SharipovaInna Borisovna Leshchinskaya
Alkaline phosphatase (APase) was isolated from the culture liquid of the streptomycin-resistant strain of Bacillus intermedius S3-19 and purified as a homogeneous preparation by ion-exchange chromatography and FPLC. Electrophoresis and gel-filtration revealed that the active enzyme is a...read more
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Ion-Exchange Chromatography Procedure
Alkaline Phosphatase Measurement
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